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MTR Antibody (PA5-114366) in IHC (P)
Immunohistochemistry analysis of MTR in paraffin-embedded human pancreatic cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in MTR polyclonal antibody (Product # PA5-114366) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with ... View More
Product Details
PA5-114366
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MTR recombinant protein (Position: V880-D1095).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2884823
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human K562 whole cell. IHC: human placenta tissue, mouse brain tissue, rat brain tissue, rat brain tissue, human pancreatic cancer tissue. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
MTR encodes the enzyme 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-methyltetrahydrofolate--homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase 1; Cobalamin-dependent methionine synthase; FLJ33168; FLJ43216; FLJ45386; Methionine synthase; MS; Vitamin-B12 dependent methionine synthase
Gene Aliases: AI894170; cblG; D830038K18Rik; HMAG; methioninesynthase; MS; MTR
UniProt ID: (Human) Q99707, (Rat) Q9Z2Q4, (Mouse) A6H5Y3
Entrez Gene ID: (Human) 4548, (Rat) 81522, (Mouse) 238505
sulfur amino acid metabolic process
nervous system development
methionine biosynthetic process
cobalamin metabolic process
axon regeneration
methylation
pteridine-containing compound metabolic process
response to axon injury
cellular response to nitric oxide
protein methylation
methionine metabolic process
tetrahydrofolate metabolic process
homocysteine metabolic process
cellular response to methionine
cellular amino acid biosynthetic process
cellular metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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