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MUT Antibody (PA5-79708) in ICC/IF
Immunocytochemistry analysis of MUT using anti-MUT antibody (Product # PA5-79708) . MUT was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MUT antibody (Product # PA5-79708) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize... View More
Product Details
PA5-79708
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MUT (33-65aa LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746823
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, huam CACO-2 whole cell, rat liver tissue, rat kidney tissue, rat heart tissue, mouse liver tissue, mouse kidney tissue, mouse heart tissue. IHC: human intestinal cancer tissue, human lung cancer tissue, human mammary cancer tissue. ICC/IF: U20S cell. Flow: A431 cell.
Target Information
The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MCM; methylmalonyl CoA mutase; methylmalonyl Coenzyme A mutase; Methylmalonyl-CoA isomerase; Methylmalonyl-CoA mutase, mitochondrial; methylmalonyl-Coenzyme A mutase; mutant methylmalonyl CoA mutase; truncated methylmalonyl CoA mutase
Gene Aliases: D230010K02Rik; MCM; MMUT; MUT
UniProt ID: (Human) P22033, (Mouse) P16332
Entrez Gene ID: (Human) 4594, (Mouse) 17850, (Rat) 688517
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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