Invitrogen

MX1 Polyclonal Antibody

MX1 Antibody in Immunocytochemistry (ICC/IF)

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MX1 Antibody (PA5-22101) in ICC/IF

Immunofluorescence analysis of MX1 was performed using 70% confluent log phase HeLa and HeLa cells treated with IFN Alpha (10 ng/mL, 16hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MX1 Polyclonal Antibody (Product # PA5-22101) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alex... View More

MX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MX1 Antibody in Immunohistochemistry (IHC)

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MX1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of MX1 using MX1 Monoclonal Antibody (Product # PA5-22101) shows increased expression of MX1 protein in cytoplasm of HeLa cell line upon treatment with IFN Alpha. {TM}

产品信息

PA5-22101

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Bovine, Human, Non-human primate, Rabbit

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 194 and 475 (P20591) of MX1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.52 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11153215

产品详细信息

Recommended positive controls: Jurkat, Raji, NCI-H929, human MX1-transfected 293T.

Predicted reactivity: Rhesus Monkey (92%), Chimpanzee (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Subviral Dense Bodies of Human Cytomegalovirus Enhance Interferon-Beta Responses in Infected Cells and Impair Progeny Production.

Viruses

Penner I,Büscher N,Krauter S,Plachter B

Published figure using MX1 polyclonal antibody (Product # PA5-22101) in Western Blot

Wed Jun 07 00:00:00 UTC 2023

The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses.

EMBO reports

Bonaventure B,Rebendenne A,Chaves Valadão AL,Arnaud-Arnould M,Gracias S,Garcia de Gracia F,McKellar J,Labaronne E,Tauziet M,Vivet-Boudou V,Bernard E,Briant L,Gros N,Djilli W,Courgnaud V,Parrinello H,Rialle S,Blaise M,Lacroix L,Lavigne M,Paillart JC,Ricci EP,Schulz R,Jouvenet N,Moncorgé O,Goujon C

Published figure using MX1 polyclonal antibody (Product # PA5-22101) in Western Blot

Mon Nov 07 00:00:00 UTC 2022

Subviral Dense Bodies of Human Cytomegalovirus Induce an Antiviral Type I Interferon Response.

Cells

Penner I,Büscher N,Dejung M,Freiwald A,Butter F,Plachter B

PA5-22101 was used in Western Blotting to investigate the impact of dense bodies on cells, in the absence of a viral infection.

Tue Dec 13 00:00:00 UTC 2022

Subviral Dense Bodies of Human Cytomegalovirus Induce an Antiviral Type I Interferon Response.

Cells

Penner I,Büscher N,Dejung M,Freiwald A,Butter F,Plachter B

PA5-22101 was used in Western Blotting to investigate the impact of dense bodies on cells, in the absence of a viral infection.

Tue Dec 13 00:00:00 UTC 2022

System-wide identification of myeloid markers of TB disease and HIV-induced reactivation in the macaque model of Mtb infection and Mtb/SIV co-infection.

Frontiers in immunology

Gough M,Singh DK,Singh B,Kaushal D,Mehra S

Published figure using MX1 polyclonal antibody (Product # PA5-22101) in Immunohistochemistry

Tue Oct 25 00:00:00 UTC 2022

SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells.

Journal of virology

Rebendenne A,Valadão ALC,Tauziet M,Maarifi G,Bonaventure B,McKellar J,Planès R,Nisole S,Arnaud-Arnould M,Moncorgé O,Goujon C

PA5-22101 was used in Western Blot to characterize in depth host cell responses against SARS-CoV-2 in primary human airway epithelia (HAE) and immortalized cell lines.

Thu Mar 25 00:00:00 UTC 2021

SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells.

Journal of virology

Rebendenne A,Valadão ALC,Tauziet M,Maarifi G,Bonaventure B,McKellar J,Planès R,Nisole S,Arnaud-Arnould M,Moncorgé O,Goujon C

PA5-22101 was used in Western Blot to characterize in depth host cell responses against SARS-CoV-2 in primary human airway epithelia (HAE) and immortalized cell lines.

Thu Mar 25 00:00:00 UTC 2021

生物信息学

蛋白别名: GTP-binding protein; IFI-78K; Interferon-induced GTP-binding protein Mx1; Interferon-induced GTP-binding protein Mx1, N-terminally processed; Interferon-induced protein p78; interferon-inducible myxovirus resistance-1 protein; interferon-inducible protein p78; Interferon-regulated resistance GTP-binding protein MxA; MX dynamin-like GTPase 1; Myxoma resistance protein 1; myxovirus (influenza virus) resistance 1, interferon-inducible protein p78; myxovirus (influenza) resistance 1, (murine homolog); Myxovirus resistance protein 1

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基因别名: IFI-78K; IFI78; MX; MX1; Mx1-b; Mx1B; MxA

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UniProt ID: (Human) P20591, (Bovine) P79135

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Entrez Gene ID: (Rabbit) 100359014, (Human) 4599, (Bovine) 280872

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功能

GTPase activity protein binding GTP binding

参与通路