Invitrogen

MYH10 Monoclonal Antibody (GT246)

MYH10 Antibody in Immunocytochemistry (ICC/IF)

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MYH10 Antibody (MA5-27767) in ICC/IF

MYH10 Monoclonal Antibody (GT246) detects MYH10 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MYH10 protein stained by MYH10 Monoclonal Antibody (GT246) (Product # MA5-27767) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

MYH10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MYH10 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

MA5-27767

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

GT246

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2735278

产品详细信息

Positive Control: HeLa, MYH10-transfected 293T, NIH-3T3, mouse brain

Predicted Reactivity: Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

MYH10 encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Atp7b-dependent choroid plexus dysfunction causes transient copper deficit and metabolic changes in the developing mouse brain.

PLoS genetics

Washington-Hughes CL,Roy S,Seneviratne HK,Karuppagounder SS,Morel Y,Jones JW,Zak A,Xiao T,Boronina TN,Cole RN,Bumpus NN,Chang CJ,Dawson TM,Lutsenko S

MA5-27767 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to identify the new role for Atp7b in developing brain and identify metabolic changes that could be exacerbated by Cu chelation therapy.

Sun Jan 01 00:00:00 UTC 2023

生物信息学

蛋白别名: Cellular myosin heavy chain, type B; flectin; MCH-B(B2); Myosin heavy chain 10; myosin heavy chain 10, non-muscle; Myosin heavy chain, non-muscle IIb; myosin heavy chain, nonmuscle type B; myosin heavy chain, nonmuscular, type B; myosin IIB; myosin, heavy polypeptide 10, non-muscle; Myosin-10; NMMHC II-b; NMMHC IIB; NMMHC-B; NMMHC-IIB; Non-muscle myosin heavy chain B; Non-muscle myosin heavy chain IIb; nonmuscle myosin; nonmuscle myosin heavy chain II-B; nonmuscle myosin heavy chain IIB; nonmuscle myosin heavy chain-B; nonmuscle myosin II heavy chain-B; OTTHUMP00000165799

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基因别名: 5730504C04Rik; 9330167F11Rik; Fltn; MCH-B; mKIAA3005; MYH10; Myhn-2; Myhn2; NMHC II-B; NMHC-B; NMHCII-B; NMMHC II-b; NMMHC-B; NMMHC-IIB; NMMHCB; SMemb

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UniProt ID: (Human) P35580, (Mouse) Q61879, (Rat) Q9JLT0

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Entrez Gene ID: (Human) 4628, (Mouse) 77579, (Rat) 79433

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功能