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MYO1D Antibody (PA5-120743) in WB
Western blot analysis of MYO1D in extracts of various cell lines. Samples were incubated with MYO1D Polyclonal antibody (Product # PA5-120743) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-120743
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 180-380 of human MYO1D (NP_001290209.1).
Conjugate
Form
Liquid
Concentration
1.276 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914315
Product Specific Information
Positive test controls include: SKOV3, Mouse lung, Mouse brain.
Immunogen sequence: HINNYLLEKS RVIVQQPGER SFHSFYQLLQ GGSEQMLRSL HLQKSLSSYN YIHVGAQLKS SINDAAEFRV VADAMKVIGF KPEEIQTVYK ILAAILHLGN LKFVVDGDTP LIENGKVVSI IAELLSTKTD MVEKALLYRT VATGRDIIDK QHTEQEASYG RDAFAKAIYE RLFCWIVTRI NDIIEVKNYD TTIHGKNTVI G
Target Information
Unconventional myosin that functions as actin-based motor protein with ATPase activity. Plays a role in endosomal protein trafficking, and especially in the transfer of cargo proteins from early to recycling endosomes. Required for normal planar cell polarity in ciliated tracheal cells, for normal rotational polarity of cilia, and for coordinated, unidirectional ciliary movement in the trachea. Required for normal, polarized cilia organization in brain ependymal epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: myo1d; myosin-I gamma; myosin-Id; protein phosphatase 1, regulatory subunit 108; Unconventional myosin-Id
Gene Aliases: 9930104H07Rik; AW544947; D11Ertd9e; KIAA0727; MYO1D; myosin-1d; myr4; PPP1R108
UniProt ID: (Human) O94832, (Mouse) Q5SSK7
Entrez Gene ID: (Human) 4642, (Mouse) 338367
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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