Invitrogen

MYO3B Polyclonal Antibody

MYO3B Antibody in Immunocytochemistry (ICC/IF)

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MYO3B Antibody (PA5-103938) in ICC/IF

Immunocytochemistry analysis of MYO3B in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MYO3B polyclonal antibody (Product # PA5-103938) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

MYO3B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-103938

应用

建议稀释比

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免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human MYO3B(Accession Q8WXR4), corresponding to amino acid residues R1260-S1310.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2853270

产品详细信息

Antibody detects endogenous levels of total MYO3B.

靶标信息

MYO3B (MYO3 beta) is a member of the Class III myosins that are actin-dependent motor proteins containing an amino-terminal kinase domain. MYO3B contains a N-terminal kinase domain, followed by motor, neck, and tail domains. The MYO3B gene generates a variety of splice variants that contain 1 or 2 calmodulin-binding (IQ) motifs in the neck domain and 1 of 3 domains in the tail domain. Northern blot analysis shows expression of a 7-kb MYO3B transcript in the human retina but not in a RPE cell line. The MYO3B gene transcript is also detected in the kidney, intestine and testis. The MYO3B gene maps to chromosome 2q31.1-q31.2 by genomic sequence alignment.

仅用于科研。不用于诊断过程。未经明确授权不得转售。