Invitrogen

MYOCD Polyclonal Antibody

查看其他6个MYOCD抗体

抗体检测数据 (12)

MYOCD Antibody in Immunocytochemistry (ICC/IF)

MYOCD Antibody in Immunocytochemistry (ICC/IF)

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MYOCD Antibody (PA5-100775) in ICC/IF

Immunocytochemistry analysis of MYOCD in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

MYOCD Antibody in Immunocytochemistry (ICC/IF)

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MYOCD Antibody in Western Blot (WB)

MYOCD Antibody in Western Blot (WB)

MYOCD Antibody in Western Blot (WB)

MYOCD Antibody in Western Blot (WB)

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MYOCD Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of MYOCD in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MYOCD in mouse thymus tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MYOCD in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MYOCD in mouse ovary tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MYOCD in rat spleen tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MYOCD in rat ovary tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MYOCD in rat brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MYOCD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MYOCD in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MYOCD Antibody in Western Blot (WB) — Western blot analysis of MYOCD in extracts from HepG2 cells (LPS 4h treatment). Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775). The lane on the left was treated with blocking peptide.

MYOCD Antibody in Western Blot (WB) — Western blot analysis of MYOCD in MCF7 cell lysate (Lane 1: treated with blocking peptide). Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775).

MYOCD Antibody in Western Blot (WB) — Western blot analysis of MYOCD in extracts from H2O2 treated Hela cells. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775).

MYOCD Antibody in Western Blot (WB) — Western blot analysis of MYOCD in rat brain. Samples were incubated with MYOCD polyclonal antibody (Product # PA5-100775).

产品信息

PA5-100775

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human MYOCD(Accession Q8IZQ8), corresponding to amino acid residues G221-P271.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2850278

产品详细信息

Antibody detects endogenous levels of total MYOCD.

靶标信息

Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Basic SAP coiled-coil transcription activator 2; Myocardin; SRF co-factor protein (cardiac and smooth muscle); SRF cofactor protein; transcription factor myocardin

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基因别名: Bsac2; BSAC2A; MYCD; MYOCD; Srfcp

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UniProt ID: (Human) Q8IZQ8, (Mouse) Q8VIM5, (Rat) Q8R5I7

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Entrez Gene ID: (Human) 93649, (Mouse) 214384, (Rat) 246297

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core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding transcription factor binding histone acetyltransferase binding histone deacetylase binding R-SMAD binding transcription coactivator activity molecular_function DNA-binding transcription factor

negative regulation of transcription from RNA polymerase II promoter regulation of cell growth by extracellular stimulus vasculogenesis response to hypoxia cardiac ventricle development positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription from RNA polymerase II promoter positive regulation of cell proliferation negative regulation of cell proliferation negative regulation of cardiac muscle cell apoptotic process negative regulation of myotube differentiation positive regulation of transforming growth factor beta receptor signaling pathway cardiocyte differentiation regulation of histone acetylation hepatic stellate cell activation vascular smooth muscle cell differentiation positive regulation of DNA binding cellular component maintenance regulation of myoblast differentiation negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle contraction lung alveolus development digestive tract development positive regulation of sequence-specific DNA binding transcription factor activity smooth muscle cell differentiation regulation of smooth muscle cell differentiation positive regulation of smooth muscle cell differentiation cardiac muscle cell differentiation ventricular cardiac muscle cell differentiation uterus development urinary bladder development cell growth involved in cardiac muscle cell development cellular response to growth factor stimulus cellular response to hypoxia ductus arteriosus closure negative regulation of beta-amyloid clearance regulation of phenotypic switching positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation positive regulation of cardiac vascular smooth muscle cell differentiation positive regulation of cardiac muscle cell differentiation negative regulation of skeletal muscle cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter heart development muscle cell differentiation