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Invitrogen
Mature Macrophage Marker Monoclonal Antibody (eBio25F9 (25F9)), eBioscience™
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Mature Macrophage Marker Antibody (14-0115-82) in Flow
Staining of cultured macrophages from peripheral blood monocytes with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human mature macrophage marker Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin APC (Product # 17-4314-82). Total viable cells were used for analysis.
产品信息
14-0115-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBio25F9 (25F9)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_953652
产品详细信息
Description: The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures. Expression is thought to correlate with endocytic macrophages and is useful in many disease models. Expression is not found on immature macrophages or monocytes or any other hematopoietic cell.
Applications Reported: This eBio25F9 (25F9) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen and paraffin embedded tissue sections.
Applications Tested: This eBio25F9 (25F9) antibody has been tested by flow cytometric analysis of human macrophages. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ED2-like antigen; Macrophages; Mature Macrophages
