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Invitrogen
Mouse IgG1/IgG2a Isotype Control Cocktail, FITC, PE
产品信息
PA5-33181
应用
建议稀释比
流式细胞分析 (Flow)
Neat
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Mouse
/ IgG1/IgG2a
分类
Cocktail
类型
Isotype Control
偶联物
形式
Lyophilized
浓度
Conc. Not Determined
纯化类型
purified
保存液
PBS with 1% BSA, 5% sucrose
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2550598
产品详细信息
PA5-33181 is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonals of isotype IgG1 and IgG2a in human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes. This antibody contains a monoclonal mixture of FITC-conjugated Mouse IgG1 RPE-conjugated Mouse IgG2a.
Reconstitute with 0.5 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
靶标信息
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: IgG2; Immunoglobulin G; Immunoglobulin G1; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG1; ImmunoglobulinG2
