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Mouse Serum Albumin Polyclonal Antibody

Mouse Serum Albumin Antibody in Western Blot (WB)

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Mouse Serum Albumin Antibody (600-401-254) in WB

Western Blot of rabbit anti-mouse serum albumin. Lane 1: molecular weight. Lane 2: mouse serum albumin. Load: 0.1 µg. Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:20,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) 30 min at 4°C. Predicted/Observed size: 69 kDa, 72 kDa for MSA. Other band(s): none.

Mouse Serum Albumin Antibody in ELISA (ELISA)

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产品信息

600-401-254

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化 (IHC)

1:500-1:2,500

酶联免疫吸附实验 (ELISA)

1:20,000-1:100,000

免疫沉淀 (IP)

1:2,000-1:10,000

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-Mouse Serum Albumin Antibody was produced by repeated immunizations with mouse serum albumin.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.

靶标信息

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。