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Human IgG4 Fc Secondary Antibody (A-10654) in WB
Western blot was performed Mouse anti-Human IgG4 Fc Secondary Antibody, HRP (Product # A-10654) and a 55 kDa band corresponding to Human IgG4 Heavy Chain was observed in IgG48203, along with cross reactivity in Mouse and Rabbit IgG but not in IgG1, IgG2, IgG3, IgA, IgM, IgE, or Mouse IgM8203. Purified protein (100 ng) of Human IgG1 (Lane 1), IgG2 (Lane 2), IgG3 (Lane 3), IgG4 (Lane 4), IgA (Lane 5), IgM (La... View More
产品信息
A-10654
应用
建议稀释比
免疫印迹 (WB)
1:500-1:2,000
免疫组化 (IHC)
1:500-1:2,000
酶联免疫吸附实验 (ELISA)
1:500-1:2,000
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Secondary Antibody
克隆号
HP6025
抗原
Human IgG4
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS
内含物
0.02% thimerosal
保存条件
-20°C
运输条件
Ambient
RRID
AB_2534054
靶标
IgG4
抗体形式
Whole Antibody
产品详细信息
Storage and reconstitution: when stored desiccated at or below -20°C, the lyophilized powder is stable for at least one year. Reconstitute using 200 µL PBS, pH 7.2, to yield a 1 mg/mL stock solution containing 0.02% thimerosal. After reconstitution, store product at 4°C.
For prolonged storage after reconstitution, add glycerol to a final concentration of 50% (v/v), aliquot, and store at or below -20°C. When stored properly, the solution is stable for approximately three months.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
