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Mre11 Antibody (600-401-872) in ChIP
Chromatin Immunoprecipitation (ChIP) using Rocklands Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked sec... View More
产品信息
600-401-872
应用
建议稀释比
已发表文章
产品规格
种属反应
Yeast
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 575-600 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.
偶联物
形式
Liquid
浓度
1.17 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against Saccharomyces cerevisiae Mre11 protein. A BLAST analysis was used to suggest that little cross reactivity should be expected with this protein from other sources.
靶标信息
MRE11 (double-strand break repair protein MRE11) is a component of the MRN complex. This complex plays a central role in double-strand break repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-stranded endonuclease activity and double-strand-specific 3'-5' exonuclease activity which are provided by MRE11. RAD50 is required to bind DNA ends and hold them in close proximity. Mutations in the gene could result in ataxia-telangiectasia-like disorder 1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Double-strand break repair protein MRE11
基因别名: MRE11; NGS1; RAD58; XRS4; YM9959.06C; YMR224C
UniProt ID: (Baker's yeast) P32829
Entrez Gene ID: (Baker's yeast) 855264
single-stranded DNA endodeoxyribonuclease activity
double-stranded telomeric DNA binding
adenylate kinase activity
nuclease activity
endonuclease activity
endodeoxyribonuclease activity
exonuclease activity
3'-5'-exodeoxyribonuclease activity
3'-5' exonuclease activity
hydrolase activity
manganese ion binding
telomeric DNA binding
single-stranded telomeric DNA binding
G-quadruplex DNA binding
binding, bridging
meiotic DNA double-strand break processing
telomere maintenance
double-strand break repair via homologous recombination
double-strand break repair via break-induced replication
DNA repair
base-excision repair
double-strand break repair
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
mitotic G2 DNA damage checkpoint
reciprocal meiotic recombination
DNA double-strand break processing involved in repair via synthesis-dependent strand annealing
ascospore formation
intra-S DNA damage checkpoint
maintenance of DNA trinucleotide repeats
meiotic DNA double-strand break formation
nucleoside monophosphate phosphorylation
regulation of transcription involved in meiotic cell cycle
meiotic cell cycle
mitochondrial double-strand break repair via homologous recombination
