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Mre11 Antibody (600-401-896) in WB
Western blot using Rocklands Affinity Purified anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 µg, 0.3 µg, 0.1 µg and 0.05 µg of purified mouse Mre11 protein, respectively. After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4°C. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at r... View More
产品信息
600-401-896
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 68-706 of mouse Mre11 protein.
偶联物
形式
Liquid
浓度
1.05 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Mre11 protein. A BLAST analysis was used to suggest cross reactivity with Mre11 protein from rat (94% homology). Based on protein sequence homology also expect partial reactivity against Mre11 homologues from macaque (88%), human (84%) and dog (82%). This sequence shows less than 75% homology to homologues from bovine, Xenopus, chicken, chimpanzee and zebrafish sources. Reactivity against homologues from other sources is not known.
靶标信息
MRE11 (double-strand break repair protein MRE11) is a component of the MRN complex. This complex plays a central role in double-strand break repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-stranded endonuclease activity and double-strand-specific 3'-5' exonuclease activity which are provided by MRE11. RAD50 is required to bind DNA ends and hold them in close proximity. Mutations in the gene could result in ataxia-telangiectasia-like disorder 1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Double-strand break repair protein MRE11; double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; Meiotic recombination 11 homolog A; mmMRE11A; MRE11 homolog 1; MRE11 homolog A
基因别名: Mre11; Mre11a; Mre11b
UniProt ID: (Mouse) Q61216
Entrez Gene ID: (Mouse) 17535
telomere maintenance
DNA metabolic process
DNA repair
base-excision repair
double-strand break repair
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
sister chromatid cohesion
mitotic G2 DNA damage checkpoint
synapsis
cell proliferation
intra-S DNA damage checkpoint
positive regulation of protein autophosphorylation
DNA duplex unwinding
negative regulation of DNA endoreduplication
positive regulation of kinase activity
negative regulation of viral entry into host cell
chromosome organization
meiotic cell cycle
nucleic acid phosphodiester bond hydrolysis
