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Musashi-2 Antibody (14-9677-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum using 10 µg/mL Mouse IgG3 Isotype Control Purified (left) or 10 µg/mL Anti-Human/Mouse Musashi-2 Purified (right) followed by Anti-Mouse IgG Biotin and DAB visualization.Nuclei are counterstained with hematoxylin.
产品信息
14-9677-82
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG3, kappa
分类
Monoclonal
类型
Antibody
克隆号
C1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572954
产品详细信息
Description: The monoclonal antibody C1 recognizes human and mouse Musashi-2 (Msi2), a highly conserved RNA-binding protein. Musashi-2 contains two RNA-recognition domains and is expressed as one of two isoforms of 36 and 37 kDa. Musashi 2 with its family member Musashi-1, are expressed at high levels in stem and tumor cells. During neural development, Musashi-2 is expressed in progenitor cells in the ventricular and subventricular zones, while postnatally, Musashi-2 expression is limited to multiple subsets of neurons and astrocytes in addition to adult stem cell populations. Functionally, Musashi-2 associates with Sox2 and is required for self-renewal of embryonic stem cells. Much like its role in maintaining stemness in neural progenitors, Musashi-2 is expressed in hematopoetic stem cells and plays a similar role in maintaining self-renewal. Musashi-2 has been found to be overexpressed in human myeloid leukemia and the levels of expression correlate with decreased survival. Musashi-2 may serve as a prognostic marker for acute myeloid leukemia (AML).
Applications Reported: This C1 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This C1 antibody has been tested immunohistochemistry on human FFPE tissue using high or low pH antigen retrieval at less than or equal to 10 µg/mL. The C1 antibody has also been tested by western blot on reduced whole mouse brain and SK-N-SH cell lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to posttranslational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events.Tissue specificity: Ubiquitous; detected at low levels.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Musashi homolog 2; musashi RNA-binding protein 2; Musashi-2; Musashi2; RNA-binding protein Musashi homolog 2
基因别名: 1700105C15Rik; AI451722; AI563628; AW489193; MSI2; MSI2H; RGD1560397
UniProt ID: (Human) Q96DH6, (Mouse) Q920Q6
Entrez Gene ID: (Human) 124540, (Mouse) 76626, (Rat) 360596
