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Myc Tag Antibody (R953-25) in ICC/IF
Immunofluorescent analysis of Myc tag was performed using 70% confluent His-H3-Myc transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myc Tag Monoclonal Antibody (Product # R953-25) at 1:500 dilution and Histone H3 antibody (Product # 711055) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight, and then labeled with Goat anti-Rabbit IgG (H+L) Highl... View More
产品信息
R953-25
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
抗原
c-Myc synthetic peptide: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
PBS
内含物
0.01% sodium azide
保存条件
4° C, store in dark
RRID
AB_2556563
产品详细信息
NOTE: R953-25 was previously sold under product #46-0307. When re-ordering, please use #R953-25.
R953-25 recognizes amino acid sequence: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-.
This antibody has been tested in IF experiments using cultured CHO (Chinese Hamster ovary) cells expressing recombinant epitope-tagged fusion proteins. For IF, dilute the FITC-conjugated antibody in PBS containing 10% fetal bovine serum (FBS). The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction.
This antibody has also been tested against purified Positope™ control protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5.
靶标信息
Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 46-0603; c Myc; c-myc epitope tag; cmyc; Myc protein; Myc Tag; R950-25; R95025
