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MyoD1 Antibody (4654-MSM11-P1ABX) in IHC (P)
Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human rhabdomyosarcoma using MyoD1 Recombinant Monoclonal Antibody (Product # 4654-MSM11-P1ABX) at 2 µg/mL in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. Secondary: HRP-polymer, 30 min. DAB, 5 min.
产品信息
4654-MSM11-P1ABX
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
rMYOD1, 6911
抗原
Recombinant fragment (around aa1-100) of human MyoD1 protein
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
靶标信息
The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: bHLHc1; Class C basic helix-loop-helix protein 1; Myf-3; Myoblast determination protein 1; Myogenic factor 3
基因别名: BHLHC1; MYF3; MYOD; MYOD1; PUM
UniProt ID: (Human) P15172
Entrez Gene ID: (Human) 4654
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
protein binding
transcription factor binding
chromatin DNA binding
ubiquitin protein ligase binding
nuclear hormone receptor binding
protein heterodimerization activity
E-box binding
basic helix-loop-helix transcription factor
regulation of alternative mRNA splicing, via spliceosome
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein phosphorylation
muscle organ development
myoblast fate determination
skeletal muscle tissue development
myoblast fusion
cellular response to starvation
myotube cell development
myotube differentiation involved in skeletal muscle regeneration
skeletal muscle cell differentiation
muscle cell fate commitment
positive regulation of skeletal muscle tissue regeneration
regulation of RNA splicing
skeletal muscle fiber adaptation
histone H3 acetylation
histone H4 acetylation
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
skeletal muscle fiber development
positive regulation of skeletal muscle fiber development
positive regulation of muscle cell differentiation
cellular response to glucocorticoid stimulus
cellular response to estradiol stimulus
cellular response to oxygen levels
positive regulation of myoblast fusion
negative regulation of myoblast proliferation
