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Myogenin Antibody (53-5643-82) in ICC/IF
Immunofluorescence analysis of MYOG was performed using 70% confluent log phase RD and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myogenin Monoclonal Antibody (F5D), Alexa Fluor™ 488, eBioscience™ (Product # 53-5643-82, 5 µg/mL) in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant ... View More
产品信息
53-5643-82
产品规格
种属反应
Dog,
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
F5D
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1907433
产品详细信息
Description: This F5D monoclonal antibody reacts with human, mouse, dog, and rat myogenin, a 34-kDa transcription factor. Expressed in skeletal and heart muscle, myogenin is a member of the MyoD family of basic-helix-loop-helix proteins, which also includes MyoD, Myf5, and MRF4. This transcription factor interacts with other helix-loop-helix proteins, which may or may not be muscle-specific. Myogenin plays a significant role in myogenic differentiation, even directing nonmuscle cells to the myogenic lineage. Transforming growth factor-beta (TGFb) and bone morphogenetic protein-2 (BMP2) inhibit myogenin transcriptional activity. Predominantly residing within the nucleus, the subcellular localization of myogenin has been shown to be dependent on differentiation status and cell density. For instance, trafficking of myogenin between the nucleus and cytoplasm has been reported during skeletal muscle differentiation to mediate transcription control.
Applications Reported: This F5D antibody has been reported for use in immunocytochemistry.
Applications Tested: This F5D antibody has been tested by immunocytochemistry of the C2C12 cell line. This can be used at less than or equal to 5-20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: bHLHc3; Class C basic helix-loop-helix protein 3; Myf-4; MYOD1-related protein; Myogenic factor 4; Myogenic factor-4; Myogenin
基因别名: BHLHC3; myf-4; MYF4; myo; MYOG
UniProt ID: (Human) P15173, (Mouse) P12979, (Rat) P20428
Entrez Gene ID: (Dog) 490224, (Human) 4656, (Mouse) 17928, (Rat) 29148
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
chromatin DNA binding
sequence-specific DNA binding
protein heterodimerization activity
E-box binding
core promoter proximal region sequence-specific DNA binding
DNA binding
protein dimerization activity
molecular_function
basic helix-loop-helix transcription factor
ossification
cell cycle
skeletal muscle tissue development
negative regulation of cell proliferation
response to gravity
positive regulation of myotube differentiation
skeletal muscle atrophy
positive regulation of muscle atrophy
regulation of skeletal muscle satellite cell proliferation
response to muscle activity involved in regulation of muscle adaptation
response to electrical stimulus involved in regulation of muscle adaptation
striated muscle atrophy
response to denervation involved in regulation of muscle adaptation
skeletal muscle cell differentiation
muscle cell fate commitment
mRNA transcription from RNA polymerase II promoter
skeletal muscle tissue regeneration
myoblast differentiation
positive regulation of myoblast differentiation
negative regulation of glycolytic process
positive regulation of transcription from RNA polymerase II promoter
skeletal muscle fiber development
positive regulation of skeletal muscle fiber development
positive regulation of muscle cell differentiation
positive regulation of cell cycle arrest
cellular response to magnetism
cellular response to lithium ion
cellular response to retinoic acid
cellular response to growth factor stimulus
cellular response to estradiol stimulus
regulation of myoblast fusion
positive regulation of myoblast fusion
positive regulation of oxidative phosphorylation
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
multicellular organism development
muscle organ development
myotube differentiation
cell differentiation
histone H3 acetylation
histone H4 acetylation
positive regulation of transcription, DNA-templated
response to muscle activity
