Invitrogen

N-cadherin Polyclonal Antibody

View all (98) N-cadherin antibodies
Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-94960

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin (701-714aa CQCDSNGDCTDVDR).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806766

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat brain tissue, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue, human SKOV3 whole cell. IHC: human tonsil tissue, mouse cardiac muscle tissue, rat cardiac muscle tissue, human liver cancer tissue. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

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Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

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UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3

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Entrez Gene ID: (Human) 1000, (Mouse) 12558, (Rat) 83501

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Function(s)
calcium ion binding protein binding beta-catenin binding protein kinase binding protein phosphatase binding protein complex binding alpha-catenin binding gamma-catenin binding nitric-oxide synthase binding enzyme binding metal ion binding
Process(es)
cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules synapse assembly regulation of signal transduction glial cell differentiation single organismal cell-cell adhesion calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration regulation of myelination regulation of protein localization regulation of Rho protein signal transduction positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis regulation of axonogenesis striated muscle cell differentiation protein heterooligomerization negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering cerebral cortex development adherens junction organization brain morphogenesis homeostasis of number of cells positive regulation of muscle cell differentiation radial glial cell differentiation neuroepithelial cell differentiation regulation of oligodendrocyte progenitor proliferation establishment of protein localization to plasma membrane
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