Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
N-cadherin Recombinant Rabbit Monoclonal Antibody (020)
FIGURE: 1 / 6
N-cadherin Antibody (MA5-29140) in ICC/IF
Immunofluorescence analysis of N-cadherin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with N-cadherin Recombinant Rabbit Monoclonal Antibody (20) (Product # MA5-29140, 1:50) in 0.1% BSA, incubated at ... View More
Product Details
MA5-29140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
020
Immunogen
Recombinant Human N-Cadherin/CD325/CDH2 Protein (Met1-Ala724)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785062
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human N-Cadherin/CD325/CDH2.
Target Information
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin
Gene Aliases: CD325; CDH2; CDHN; CDw325; NCAD
UniProt ID: (Human) P19022
Entrez Gene ID: (Human) 1000
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
glial cell differentiation
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
cerebral cortex development
regulation of myelination
adherens junction organization
regulation of Rho protein signal transduction
positive regulation of MAPK cascade
cell-cell adhesion mediated by cadherin
blood vessel morphogenesis
brain morphogenesis
homeostasis of number of cells
regulation of axonogenesis
striated muscle cell differentiation
positive regulation of muscle cell differentiation
protein heterooligomerization
radial glial cell differentiation
neuroepithelial cell differentiation
regulation of oligodendrocyte progenitor proliferation
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
neuroligin clustering involved in postsynaptic membrane assembly
neuronal stem cell population maintenance
regulation of postsynaptic density protein 95 clustering
positive regulation of synaptic vesicle clustering
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support