Invitrogen

N-cadherin Monoclonal Antibody (8C11)

N-cadherin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 12

N-cadherin Antibody (MA1-2002) in ICC/IF

Immunofluorescent analysis of N-Cadherin in SHSY5Y cells (human neuroblast). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) an N-Cadherin monoclonal antibody (Product # MA1-2002) at a dilution of 1... View More

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

N-cadherin Antibody in Western Blot (WB)

N-cadherin Antibody in Immunoprecipitation (IP)

Product Image Gallery

产品信息

MA1-2002

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

1:50-1:200

免疫沉淀 (IP)

5 µg

产品规格

种属反应

Hamster, Human, Mouse, Rabbit

已发表种属

Avian, Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

8C11

抗原

Bacterial fusion protein corresponding to the extracellular domain of N-cadherin.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_326076

产品详细信息

MA1-2002 detects N-Cadherin from human neuronal samples, including SHSY5Y cells. MA1-2002 also detects N-Cadherin in rabbit and hamster brain lysates. MA1-2002 does not detect N-Cadherin in lysates from mouse, rat, pig and cow brain. This antibody is not suitable for mouse C2C12 cell lysates in Western blot analysis.

MA1-2002 has been successfully used in Western blot, Immunoprecipitation, IHC (P) and Immunofluorescence applications. By Western blot, this antibody detects N-Cadherin at 130 kDa. This antibody is not suitable for frozen tissues in immunohistochemistry applications.

The MA1-2002 immunogen is a bacterial fusion protein to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino acid residues 92-593 from human N-cadherin).

靶标信息

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Transforming Growth Factor Beta and Epithelial to Mesenchymal Transition Alter Homologous Recombination Repair Gene Expression and Sensitize BRCA Wild-Type Ovarian Cancer Cells to Olaparib.

Cancers

Roberts CM,Rojas-Alexandre M,Hanna RE,Lin ZP,Ratner ES

MA1-2002 was used in Western Blotting to find that TGFβ and EMT resulted in the downregulation of genes responsible for homologous recombination (HR) and sensitized cells to olaparib.

Tue Aug 01 00:00:00 UTC 2023

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

The Journal of biological chemistry

Tsai SJ,Atai NA,Cacciottolo M,Nice J,Salehi A,Guo C,Sedgwick A,Kanagavelu S,Gould SJ

Published figure using N-cadherin monoclonal antibody (Product # MA1-2002) in Western Blot

Mon Nov 01 00:00:00 UTC 2021

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

The Journal of biological chemistry

Tsai SJ,Atai NA,Cacciottolo M,Nice J,Salehi A,Guo C,Sedgwick A,Kanagavelu S,Gould SJ

Published figure using N-cadherin monoclonal antibody (Product # MA1-2002) in Western Blot

Mon Nov 01 00:00:00 UTC 2021

The Motility and Mesenchymal Features of Breast Cancer Cells Correlate with the Levels and Intracellular Localization of Transglutaminase Type 2.

Cells

Bianchi N,Brugnoli F,Grassilli S,Bourgeois K,Keillor JW,Bergamini CM,Aguiari G,Volinia S,Bertagnolo V

MA1-2002 was used in Western Blot to investigate motility in breast cancer cell lines in association with the expression of Transglutaminase type 2 as well as upon the administration of Doxorubicin, an active cytotoxic agent that is employed in chemotherapy.

Sat Nov 06 00:00:00 UTC 2021

A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion.

Nature cell biology

Labernadie A,Kato T,Brugués A,Serra-Picamal X,Derzsi S,Arwert E,Weston A,González-Tarragó V,Elosegui-Artola A,Albertazzi L,Alcaraz J,Roca-Cusachs P,Sahai E,Trepat X

MA1-2002 was used in Immunohistochemistry to investigate the interactions between cancer-associated fibroblasts and cancer cells which drive cooperative tumour invasion.

Wed Mar 01 00:00:00 UTC 2017

Control of cell-cell forces and collective cell dynamics by the intercellular adhesome.

Nature cell biology

Bazellières E,Conte V,Elosegui-Artola A,Serra-Picamal X,Bintanel-Morcillo M,Roca-Cusachs P,Muñoz JJ,Sales-Pardo M,Guimerà R,Trepat X

MA1-2002 was used in immunohistochemistry to elucidate the contributions of cell-cell adhesion proteins to intercellular force and epithelial tissue dynamics.

Wed Apr 01 00:00:00 UTC 2015

Distinct PTPmu-associated signaling molecules differentially regulate neurite outgrowth on E-, N-, and R-cadherin.

Molecular and cellular neurosciences

Oblander SA,Brady-Kalnay SM

MA1-2002 was used in western blot to investigate the effects of PTPmu on cadherin-dependent neurite outgrowth

Sat May 01 00:00:00 UTC 2010

N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.

The Journal of biological chemistry

Wahl JK,Kim YJ,Cullen JM,Johnson KR,Wheelock MJ

MA1-2002 was used in western blot to investigate N-cadherin processing by proteolysis and the formation of N-cadherin-catenin complexes

Fri May 09 00:00:00 UTC 2003

生物信息学

蛋白别名: cadherin 2, type 1; cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

查看更多收起

基因别名: CD325; CDH2; CDHN; CDw325; I79_014914; N-cadherin; NCAD

查看更多收起

UniProt ID: (Human) P19022, (Mouse) P15116