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N-cadherin Antibody (MA5-16324) in IHC (P)
Immunohistochemical analysis of Cadherin-N/N-Cadherin using anti-Cadherin-N/N-Cadherin Monoclonal Antibody (Product # MA5-16324) in Mesothelioma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
产品信息
MA5-16324
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
SP90
抗原
Synthetic peptide corresponding to C-terminus of human cadherin-N protein.
偶联物
形式
Liquid
浓度
0.005 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.6, with 1% BSA
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537843
产品详细信息
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is mesothelioma.
靶标信息
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin
基因别名: CD325; CDH2; CDHN; CDw325; NCAD
UniProt ID: (Human) P19022
Entrez Gene ID: (Human) 1000
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
glial cell differentiation
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
cerebral cortex development
regulation of myelination
adherens junction organization
regulation of Rho protein signal transduction
positive regulation of MAPK cascade
cell-cell adhesion mediated by cadherin
blood vessel morphogenesis
brain morphogenesis
homeostasis of number of cells
regulation of axonogenesis
striated muscle cell differentiation
positive regulation of muscle cell differentiation
protein heterooligomerization
radial glial cell differentiation
neuroepithelial cell differentiation
regulation of oligodendrocyte progenitor proliferation
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
neuroligin clustering involved in postsynaptic membrane assembly
neuronal stem cell population maintenance
regulation of postsynaptic density protein 95 clustering
positive regulation of synaptic vesicle clustering
