Invitrogen

N4BP1 Polyclonal Antibody

N4BP1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

N4BP1 Antibody (PA5-117135) in ICC/IF

Immunofluorescence analysis of N4BP1 in U2OS cells. Samples were incubated with N4BP1 Polyclonal antibody (Product # PA5-117135) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Product Image Gallery

产品信息

PA5-117135

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 190-590 of mouse N4BP1 (NP_0850402)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.91 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2901765

产品详细信息

Positive Samples: Jurkat, 293T, Mouse brain, Mouse pancreas

Immunogen sequence: TQGEESLFET DDDVITVGDV RPPEYTQSAA TGPSSARDEV VVQEDSRNKA RTPVSELTKH MDTVFSSSPD VLFVPVNGLS PDEDALSKDR VCHKRRSSDT EERHTKKQFS LENVPEGELL PDGKGSAGNE VIDLSDPASN STNLSPDGKD TTEEMEYNIL VNFFKTMGYS QEIVEKVIRE YGPSTEPLLL LEEIEKENKR LQEDRDFPPC TVYPDASQSR NAGVGSTTNE LTADSTPKKA QSHTEQSMVE RFSQLPFKDS KHCTSNCKVN SFRTVPVGQK QEIWGSKQNS SCTVDLETDG HSASAASASP KDISFVSRGA SGHQQRNPAF PENGFQQQTE PLLPNNTKPA CEKRSGSCSS PQPKPNYPPL SPPLPLPQLL PSVTEARLGG SSDHIDSSVT G

靶标信息

Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

仅用于科研。不用于诊断过程。未经明确授权不得转售。