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N6-Methyladenosine (m6A) Antibody (MA5-44938) in DB
Dot blot analysis of m6A in HeLa cells. Loading: Total RNA extracted from HeLa. Blocking Buffer: 5% NFDM/TBST. Incubation time and temperature: 1 hours at room temperature. Samples were incubated in m6A Monoclonal antibody (Product # MA5-44938) using a dilution of 2 µg/mL (Dilution Buffer: Primary Antibody Dilution Buffer form Beyotime) Overnight at 4 &(8)451; followed by Goat anti-mouse IgG-HRP at a diluti... View More
产品信息
MA5-44938
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
A8F2
抗原
m6A Small Molecule conjugated to OVA.
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931394
产品详细信息
Application notes: Indirect ELISA
靶标信息
N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C11H15N5O4; m6A
