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NAIP Antibody (PA5-115614) in ICC/IF
Immunocytochemistry analysis of NAIP in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115614) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115614
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NAIP(Accession Q13075), corresponding to amino acid residues A302-A352.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900249
Product Specific Information
Antibody detects endogenous levels of total NAIP.
Target Information
Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR); these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules (reviewed in 3). Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: baculoviral IAP repeat-containing 1a; Baculoviral IAP repeat-containing protein 1; Baculoviral IAP repeat-containing protein 1a; FLJ18088; FLJ42520; FLJ58811; Neuronal apoptosis inhibitory protein; Neuronal apoptosis inhibitory protein 1; NLR family, apoptosis inhibitory protein; nucleotide-binding oligomerization domain, leucine rich repeat and BIR domain containing 1; psi neuronal apoptosis inhibitory protein
Gene Aliases: AV364616; BIRC1; Birc1a; D13Lsd1; NAIP; Naip-rs1; Naip1; NLRB1; psiNAIP
UniProt ID: (Human) Q13075, (Mouse) Q9QWK5
Entrez Gene ID: (Human) 4671, (Mouse) 17940
apoptotic process
inflammatory response
nervous system development
regulation of signal transduction
protein ubiquitination
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to amino acid
negative regulation of neuron apoptotic process
innate immune response
response to axon injury
negative regulation of peptidase activity
cellular response to estrogen stimulus
inhibition of cysteine-type endopeptidase activity involved in apoptotic process
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