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NAMPT Antibody (500-P222-1MG) in WB
Western Blot: To detect Human Visfatin by Western Blot analysis NAMPT Polyclonal Antibody (Product # 500-P222-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Visfatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P222-1MG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human Visfatin
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929882
产品详细信息
AA Sequence of recombinant protein: MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Visfatin. Anti-Human Visfatin-specific antibody was purified by affinity chromatography employing an immobilized Human Visfatin matrix.
Sandwich ELISA: To detect Human Visfatin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Visfatin (500-P222Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Visfatin.
Western Blot: To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Visfatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp666B131; EC 2.4.2.12; MGC117256; NAmPRTase; Nicotinamide phosphoribosyltransferase; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; Visfatin
基因别名: 1110035O14Rik; NAMPT; PBEF; PBEF1; VF; VISFATIN
UniProt ID: (Human) P43490
Entrez Gene ID: (Human) 10135
nicotinamide metabolic process
signal transduction
cell-cell signaling
female pregnancy
circadian rhythm
positive regulation of cell proliferation
insulin receptor signaling pathway
NAD biosynthetic process
response to organic cyclic compound
circadian regulation of gene expression
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of nitric-oxide synthase biosynthetic process
adipose tissue development
