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NASP Antibody (PA5-106317) in ICC/IF
Immunofluorescent analysis of NASP in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and NASP polyclonal antibody (Product # PA5-106317) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-106317
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NASP(Accession P49321), corresponding to amino acid residues S677-P727.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2853994
产品详细信息
Antibody detects endogenous levels of total NASP.
靶标信息
NASP encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp547F162; FLJ31599; FLJ35510; histone H1-binding protein; MGC19722; MGC20372; MGC2297; NASP; NASP histone chaperone; nasp {ECO:0000312|EMBL:AAH81913.1}; Nuclear autoantigenic sperm protein; nuclear autoantigenic sperm protein (histone-binding); somatic histone binding protein NASP
基因别名: 5033430J04Rik; AI131596; AI317140; D4Ertd767e; Epcs32; FLB7527; HMDRA1; NASP; Nasp-T; PRO1999
UniProt ID: (Human) P49321, (Rat) Q66HD3, (Mouse) Q99MD9
Entrez Gene ID: (Human) 4678, (Rat) 298441, (Mouse) 50927
