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NBS1 Antibody (702670) in ICC/IF
For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous NBS1 using Anti- NBS1 Recombinant Rabbit Monoclonal Antibody (Product # 702670, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of NBS1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938)... View More
产品信息
702670
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
9H4L65
抗原
Protein corresponding to human NBS1 (aa400-aa754)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2716884
产品详细信息
This antibody is predicted to react with Monkey, Bovine, Horse, Sheep
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT V1; AT V2; Cell cycle regulatory protein p95; FLJ10155; hNbs1; MGC87362; Nibrin; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome protein 1; OTTHUMP00000204088; p95 protein of the MRE11/RAD50 complex; p95-NBS1
基因别名: AT-V1; AT-V2; ATV; NBN; NBS; NBS1; P95
UniProt ID: (Human) O60934
Entrez Gene ID: (Human) 4683
DNA damage checkpoint
telomere maintenance
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
blastocyst growth
DNA replication
double-strand break repair
double-strand break repair via nonhomologous end joining
cell cycle arrest
mitotic cell cycle checkpoint
mitotic G2 DNA damage checkpoint
cell proliferation
regulation of DNA-dependent DNA replication initiation
DNA damage response, signal transduction by p53 class mediator
telomeric 3' overhang formation
positive regulation of protein autophosphorylation
positive regulation of telomere maintenance
DNA duplex unwinding
positive regulation of kinase activity
isotype switching
neuromuscular process controlling balance
meiotic cell cycle
intrinsic apoptotic signaling pathway
regulation of signal transduction by p53 class mediator
negative regulation of telomere capping
