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NCoR2 Antibody (MA1-843) in WB
Western blot analysis of NCoR2 was performed by loading 25 µg of Hela (lane 1) and Neuro-2a (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a NCoR2 monoclonal antibody (Product # MA1-843) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Prod... View More
产品信息
MA1-843
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Non-human primate
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
1542
抗原
Synthetic peptide corresponding to residues C G G A(2016) S A S D P H R E K T Q(2027) of human SMRT.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_325445
产品详细信息
MA1-843 detects silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) from human cells and tissues.
MA1-843 has been successfully used in Western blotting and immunohistochemical procedures. By Western blot, this antibody detects ~250 kDa and ~220 kDa proteins representing endogenous levels of both known isoforms of SMRT in HeLa cell extracts. This antibody does not react with mouse tissue in Western blot applications.
The MA1-843 immunogen is a synthetic peptide corresponding to residues C G G A(2016) S A S D P H R E K T Q(2027) of human SMRT. MA1-843 immunizing peptide (Cat. # PEP-043) is available for use in neutralization and control experiments.
靶标信息
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CTG repeat protein 26; N-CoR2; nuclear receptor corepressor; Nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; SMAP270; SMRT; T3 receptor-associating factor; Thyroid-, retinoic-acid-receptor-associated corepressor; TRAC
基因别名: CTG26; N-CoR2; NCOR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1
UniProt ID: (Human) Q9Y618
Entrez Gene ID: (Human) 9612
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
cellular lipid metabolic process
regulation of cellular ketone metabolic process by negative regulation of transcription from RNA polymerase II promoter
negative regulation of production of miRNAs involved in gene silencing by miRNA
