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NDUFA4 Antibody (PA5-118589) in ICC/IF
Immunocytochemical analysis of NDUFA4 in HepG2 cells using a NDUFA4 polyclonal antibody (Product # PA5-118589). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with the primary antibody and mouse anti-beta tubulin antibody at a dilution of 1:200 for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear ... View More
产品信息
PA5-118589
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NDUFA4(Accession O00483), corresponding to amino acid residues N48-F81.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2903089
产品详细信息
Antibody detects endogenous levels of total NDUFA4.
靶标信息
Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance. {ECO:0000269; PubMed:22902835}.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CI-MLRQ; Complex I 9kDa subunit; Complex I-MLRQ; Cytochrome c oxidase subunit NDUFA4; EC 1.6.5.3; EC 1.6.99.3; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4 (9 kDa, MLRQ); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4; NADH-ubiquinone oxidoreductase MLRQ subunit
基因别名: CI-9k; CI-MLRQ; MLRQ; NDUFA4
UniProt ID: (Human) O00483, (Mouse) Q62425
Entrez Gene ID: (Human) 4697, (Rat) 681024, (Mouse) 17992
