Invitrogen

NEFL Monoclonal Antibody (2F11)

产品信息

MA1-06803

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:25-1:200

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免疫组化（冰冻） (IHC (F))

1:25-1:200

免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human

已发表种属

Avian, Rabbit, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2F11

抗原

Human neurofilament preparation

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_560286

产品详细信息

MA1-06803 detects neurofilament 70 kDa in human samples.

MA1-06803 has sucessfully been used in Western blotting, and immunohistochemistry procedures. By Western blot it detects a 70 kDa band representing neurofilament ~70 kDa.

The MA1-06803 immunogen is a human neurofilament preparation.

靶标信息

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Protective distal side-to-side neurorrhaphy in proximal nerve injury-an experimental study with rats.

Acta neurochirurgica

Rönkkö H,Göransson H,Taskinen HS,Paavilainen P,Vahlberg T,Röyttä M

MA1-06803 was used in Immunocytochemistry to examine the effect of protective side-to-side anastomosis on nerve and end organ regeneration in proximal nerve injury model.

Mon Apr 01 00:00:00 UTC 2019

Nerve Tissue Prefabrication Inside the Rat Femoral Bone: Does It Work?

Turkish neurosurgery

Ozbek Z,Kocman AE,Ozatik O,Soztutar E,Ozkara E,Kose A,Arslantas A,Cetin C

MA106803 was used in immunohistochemistry - paraffin section to test if nerve regeneration can be induced in the tubular bone between distal and proximal cut nerve ends

Tue Feb 27 00:00:00 UTC 2018

Promotional effects of vasoactive intestinal peptide on the development of rodent Hertwig's epithelial root sheath.

Congenital anomalies

Xu J,Kawashima N,Fujiwara N,Harada H,Ota MS,Suda H

MA1-06803 was used in immunohistochemistry to study the potential role of vasoactive intestinal peptide in rodent Hertwig's epithelial root sheath development

Sat Sep 01 00:00:00 UTC 2012

Free radical damage as a biomarker of bladder dysfunction after partial outlet obstruction and reversal.

BJU international

Lin WY,Guven A,Juan YS,Neuman P,Whitbeck C,Chichester P,Kogan B,Levin RM,Mannikarottu A

MA1-06803 was used in immunohistochemistry to study protein carbonylation and nitrotyrosination as a biomarker of dysfunction after partial bladder outlet obstruction.

Sat Mar 01 00:00:00 UTC 2008

Effects of L-arginine and L-NAME on chronic partial bladder outlet obstruction in rabbit.

American journal of physiology. Regulatory, integrative and comparative physiology

Lin WY,Levin RM,Chichester P,Leggett R,Juan YS,Johnson A,Neumann P,Whitbeck C,Guven A,Kogan B,Mannikarottu A

MA1-06803 was used in immunohistochemistry to investigate the impact of nitric oxide synthase and blook flow on the severity of damages caused by acute and chronic partial bladder outlet obstruction

Sat Dec 01 00:00:00 UTC 2007

L-NAME, a nitric oxide synthase inhibitor, diminishes oxidative damage in urinary bladder partial outlet obstruction.

American journal of physiology. Renal physiology

Conners W,Whitebeck C,Chicester P,Legget R,Lin AD,Johnson A,Kogan B,Levin R,Mannikarottu A

MA1-06803 was used in immunohistochemistry to investigate the effect of the nitric oxide synthase inhibitor L-NAME on oxidative damage

Wed Feb 01 00:00:00 UTC 2006

Electrophysiological measurements in three-dimensional in vivo-mimetic organotypic cell cultures: preliminary studies with hen embryo brain spheroids.

Neuroscience letters

Uroukov IS,Ma M,Bull L,Purcell WM

MA1-06803 was used in immunohistochemistry to investigate the electrophysiological properties of three-dimensional hen embryo brain spheroids

Mon Aug 14 00:00:00 UTC 2006

生物信息学

蛋白别名: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

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基因别名: CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

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UniProt ID: (Human) P07196

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Entrez Gene ID: (Human) 4747

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功能

Ras guanyl-nucleotide exchange factor activity structural constituent of cytoskeleton protein binding protein C-terminus binding protein domain specific binding protein binding, bridging identical protein binding phospholipase binding protein heterodimerization activity

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