Invitrogen

NEFM Monoclonal Antibody (RNF 406)

NEFM Antibody in Immunohistochemistry (Frozen) (IHC (F))

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NEFM Antibody (OMA1-06111) in IHC (F)

Immunohistochemistry analysis of NEFM in frozen sections of rat brain tissue using a NEFM Monoclonal Antibody (RNF 406) (Product # OMA1-06111) at a dilution of 1:100.

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NEFM Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. The protein expression was observed in HEK-293 and not in HeLa and Hep G2 using NEFM Polyclonal Antibody (Product # OMA1-06111) in western blot. {RE}

产品信息

OMA1-06111

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫组化（冰冻） (IHC (F))

1:50-1:100

产品规格

种属反应

Bovine, Guinea pig, Hamster, Human, Non-human primate, Rabbit, Rat, Xenopus

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

RNF 406

抗原

Neurofilament preparation of calf brain tissue

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_325535

产品详细信息

OMA1-06111 specifically detects the phosphorylated 160 kDa neurofilament from human, guinea pig, hamster, non-human primate, rabbit and xenopus tissues.

OMA1-06111 has been successfully used in Western blot and immunohistochemistry (frozen) procedures. By Western blot, this antibody detects an ~160 kDa protein representing the phosphorylated form of the 160 kDa neurofilament in human brain extract.

OMA1-06111 antigen is cytoskeletal preparation of calf brain tissue.

靶标信息

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 160 kDa neurofilament protein; major neuronal intermediate filament subunit; medium neurofilament subunit; middle molecular weight neurofilament protein NF-M(2); Neurofilament 3; neurofilament 3, medium; neurofilament M subunit; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neuronal intermediate filament; NF-M; NMC

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基因别名: nef-m; NEF3; NEFM; nefm-a; nefm-b; NF-M; NFM; XELAEV_18020229mg

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UniProt ID: (Human) P07197, (Rat) P12839