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NEK2 Antibody (600-401-452) in WB
Western Blot of Rabbit Anti-NEK2 Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (50ng). Lane 3: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (20ng). Lane 4: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (20ng). Lane 5: Jurkat cell lysate (10µg) (p/n W09-001-370). Primary Antibody: Anti-NEK2 at 1µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 30mins at RT. Block:... View More
产品信息
600-401-452
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein.
偶联物
形式
Liquid
浓度
1.1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee. Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.
靶标信息
NEK2 is involved in cell cycle regulation and is essential for faithful chromosome segregation. NEK2 is a gene that encodes for a serine/threonine-protein kinase. This protein plays a crucial role in the regulation of mitosis and is primarily located in the centrosome. During the G1 phase, the protein is undetectable, but its levels progressively increase throughout the S phase, reaching their peak in late G2 phase. The gene has several alternatively spliced transcript variants that encode different isoforms with distinct C-termini. NEK2 is associated with several diseases, including Retinitis Pigmentosa 67 and Retinitis Pigmentosa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HSPK 21; NEK 2; NEK 2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NIMA-related kinase 2; NimA-related protein kinase 2; NLK 1; protein phosphatase 1, regulatory subunit 111; Serine/threonine-protein kinase Nek2
基因别名: HsPK21; NEK2; NEK2A; NLK1; PPP1R111; RP67
UniProt ID: (Human) P51955
Entrez Gene ID: (Human) 4751
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
blastocyst development
protein phosphorylation
chromosome segregation
mitotic nuclear division
regulation of mitotic nuclear division
positive regulation of telomere maintenance via telomerase
negative regulation of DNA binding
regulation of mitotic centrosome separation
protein autophosphorylation
spindle assembly
centrosome separation
cell division
meiotic cell cycle
positive regulation of telomerase activity
regulation of attachment of spindle microtubules to kinetochore
mitotic spindle assembly
negative regulation of centriole-centriole cohesion
positive regulation of telomere capping
