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NEK4 Antibody (PA5-21458) in ICC/IF
NEK4 Polyclonal Antibody detects NEK4 protein at nucleus by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 15 min. Green: NEK4 protein stained by NEK4 Polyclonal Antibody (Product # PA5-21458) diluted at 1:500. Blue: Hoechst 33343 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope].
产品信息
PA5-21458
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 241 and 504 of NEK4 (Uniprot ID#P51957)
偶联物
形式
Liquid
浓度
0.37 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_11151938
产品详细信息
Recommended positive controls: A431, H1299, U87-MG, SK-N-SH, IMR32, SK-N-AS.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
NEK4 is a serine/threonine kinase involved in signal transduction and cellular communication. It is proposed to act exclusively upon threonine residues and is present in most primary carcinomas. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Never in mitosis A-related kinase 4; NIMA (never in mitosis gene a)-related kinase 4; NIMA-related kinase 4; NimA-related protein kinase 4; serine/threonine kinase 2; serine/threonine protein kinase-2; Serine/threonine-protein kinase 2; Serine/threonine-protein kinase Nek4; serine/threonine-protein kinase Nek4 variant 2; Serine/threonine-protein kinase NRK2
基因别名: NEK4; NRK2; pp12301; STK2
UniProt ID: (Human) P51957
Entrez Gene ID: (Human) 6787
mitotic cell cycle
protein phosphorylation
cellular response to DNA damage stimulus
mitotic nuclear division
signal transduction by protein phosphorylation
positive regulation of transcription, DNA-templated
cell division
regulation of replicative cell aging
regulation of cellular senescence
regulation of response to DNA damage stimulus
