Invitrogen

NF-H Polyclonal Antibody

NF-H Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NF-H Antibody (PA1-10002) in IHC (P)

Immunohistological analysis of NR-H in rat cerebellum. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained with an NF-H polyclonal antibody (Product # PA1-10002) at a dilution of 1:5,000 as seen in red, and costained with a GFAP polyclonal antibody (Product # PA1-... View More

NF-H Antibody in Immunohistochemistry (Frozen) (IHC (F))

NF-H Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

NF-H Antibody in Immunocytochemistry (ICC/IF)

NF-H Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-10002

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:20,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

1:20,000

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免疫组化（石蜡） (IHC (P))

1:5,000

免疫组化（冰冻） (IHC (F))

1:2,000

免疫细胞化学 (ICC/IF)

1:20,000

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Bovine, Dog, Horse, Human, Mouse, Pig, Rat

已发表种属

Human, Mouse

宿主/亚型

Chicken / IgY

分类

Polyclonal

类型

Antibody

抗原

Native NF-H purified from bovine spinal cord.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

PBS

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1077155

产品详细信息

This antibody reacts primarily with the phosphorylated axonal form of NF-H and shows some cross-reactivity with phosphorylated NF-M, which has similar phosphorylation sites to NF-H.

靶标信息

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Specific regulation of mechanical nociception by Gβ5 involves GABA-B receptors.

JCI insight

Pandey M,Zhang JH,Adikaram PR,Kittock C,Lue N,Awe A,Degner K,Jacob N,Staples J,Thomas R,Kohnen AB,Ganesan S,Kabat J,Chen CK,Simonds WF

PA1-10002 was used in Immunohistochemistry-immunofluorescence to suggest that the targeted inhibition of Gβ5 function in Rgs7+ sensory neurons might provide specific relief for mechanical allodynia, including that contributing to chronic neuropathic pain, without reliance on exogenous opioids.

Mon Jul 10 00:00:00 UTC 2023

Myelination, axonal loss and Schwann cell characteristics in axonal polyneuropathy compared to controls.

PloS one

Placheta-Györi E,Brandstetter LM,Zemann-Schälss J,Wolf S,Radtke C

PA1-10002 was used in Immunohistochemistry and Immunocytochemistry to provide quantitative data of axonal loss, reduced myelination and Schwann cell dysfunction of polyneuropathy patients compared to neurologically healthy controls.

Thu Jan 06 00:00:00 UTC 2022

Myelination, axonal loss and Schwann cell characteristics in axonal polyneuropathy compared to controls.

PloS one

Placheta-Györi E,Brandstetter LM,Zemann-Schälss J,Wolf S,Radtke C

Thu Jan 06 00:00:00 UTC 2022

Myelination, axonal loss and Schwann cell characteristics in axonal polyneuropathy compared to controls.

PloS one

Placheta-Györi E,Brandstetter LM,Zemann-Schälss J,Wolf S,Radtke C

Thu Jan 06 00:00:00 UTC 2022

Epitope-preserving magnified analysis of proteome (eMAP).

Science advances

Park J,Khan S,Yun DH,Ku T,Villa KL,Lee JE,Zhang Q,Park J,Feng G,Nedivi E,Chung K

Published figure using NF-H polyclonal antibody (Product # PA1-10002) in Immunocytochemistry

Fri Nov 12 00:00:00 UTC 2021

Epitope-preserving magnified analysis of proteome (eMAP).

Science advances

Park J,Khan S,Yun DH,Ku T,Villa KL,Lee JE,Zhang Q,Park J,Feng G,Nedivi E,Chung K

Published figure using NF-H polyclonal antibody (Product # PA1-10002) in Immunocytochemistry

Fri Nov 12 00:00:00 UTC 2021

Nano-in-Micro-Particles Consisting of PLGA Nanoparticles Embedded in Chitosan Microparticles via Spray-Drying Enhances Their Uptake in the Olfactory Mucosa.

Frontiers in pharmacology

Spindler LM,Feuerhake A,Ladel S,Günday C,Flamm J,Günday-Türeli N,Türeli E,Tovar GEM,Schindowski K,Gruber-Traub C

Published figure using NF-H polyclonal antibody (Product # PA1-10002) in Immunocytochemistry

Tue Sep 21 00:00:00 UTC 2021

Endoplasmic reticulum stress in the dorsal root ganglia regulates large-conductance potassium channels and contributes to pain in a model of multiple sclerosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yousuf MS,Samtleben S,Lamothe SM,Friedman TN,Catuneanu A,Thorburn K,Desai M,Tenorio G,Schenk GJ,Ballanyi K,Kurata HT,Simmen T,Kerr BJ

PA1-10002 was used in Immunohistochemistry to investigate whether endoplasmic reticulum (ER) stress in dorsal root ganglia (DRG) contributes to pain hypersensitivity in the experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis.

Tue Sep 01 00:00:00 UTC 2020

Endoplasmic reticulum stress in the dorsal root ganglia regulates large-conductance potassium channels and contributes to pain in a model of multiple sclerosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yousuf MS,Samtleben S,Lamothe SM,Friedman TN,Catuneanu A,Thorburn K,Desai M,Tenorio G,Schenk GJ,Ballanyi K,Kurata HT,Simmen T,Kerr BJ

Tue Sep 01 00:00:00 UTC 2020

Sensory Neurons of the Dorsal Root Ganglia Become Hyperexcitable in a T-Cell-Mediated MOG-EAE Model of Multiple Sclerosis.

eNeuro

Yousuf MS,Noh MC,Friedman TN,Zubkow K,Johnson JC,Tenorio G,Kurata HT,Smith PA,Kerr BJ

PA1-10002 was used in Western Blotting to study the immune mediated pathophysiology of pain in multiple sclerosis.

Thu Mar 05 00:00:00 UTC 2020

Murine Retinal Citrullination Declines With Age and is Mainly Dependent on Peptidyl Arginine Deiminase 4 (PAD4).

Investigative ophthalmology & visual science

Hollingsworth TJ,Radic MZ,Beranova-Giorgianni S,Giorgianni F,Wang Y,Iannaccone A

PA1-10002 was used in Western Blotting to report on the expression levels and patterns of PAD2, PAD4 and retinal citrullination in the murine retina with age.

Wed Aug 01 00:00:00 UTC 2018

生物信息学

蛋白别名: 200 kDa neurofilament protein; heavy neurofilament protein; intermediate filament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC

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基因别名: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH

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UniProt ID: (Human) P12036, (Mouse) P19246

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Entrez Gene ID: (Dog) 442940, (Human) 4744, (Rat) 24587, (Mouse) 380684

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