Invitrogen

NF-H Recombinant Rabbit Monoclonal Antibody (4H6L2)

NF-H Antibody in Immunocytochemistry (ICC/IF)

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NF-H Antibody (701993) in ICC/IF

For immunofluorescence analysis, retinoic acid (10 um, 5 days) + BDNF (50 ng, 3 days) treated SH-SY5Y cells were fixed and permeabilized for detection of endogenous Neurofilament-H using Anti-Neurofilament H/NF-H, Phosphorylated Recombinant Rabbit Monoclonal Antibody (Product # 701993, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Neurofilament-H prote... View More

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NF-H Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence of NEFH using Anti-NEFH Recombinant Rabbit Monoclonal Antibody (Product # 701993), shows increased expression of NEFH in SH-SY5Y upon BDNF treatment. {TM}

产品信息

701993

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4H6L2

抗原

Peptide corresponding to Human NEFH (aa 631-643)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2633046

产品详细信息

This antibody is predicted to react with Monkey, Cat and Bovine.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 200 kDa neurofilament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC

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基因别名: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH

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UniProt ID: (Human) P12036, (Mouse) P19246

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Entrez Gene ID: (Human) 4744, (Rat) 24587, (Mouse) 380684

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功能