Invitrogen

NF1 Monoclonal Antibody (McNFn27a)

NF1 Antibody in Immunocytochemistry (ICC/IF)

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NF1 Antibody (MA1-084) in ICC/IF

Immunofluorescence analysis of Neurofibromin was performed using 70% confluent log phase Neuro-2A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NF1 (McNFn27a) Mouse Monoclonal Antibody (Product # MA1-084) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More

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产品信息

MA1-084

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5-10 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

亲和纯化 (AP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

McNFn27a

抗原

Synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_560294

产品详细信息

MA1-084 detects neurofibromin in human, rat and mouse cells.

MA1-084 has been successfully used in Western blot, procedures. By Western blot, this antibody detects a ~230-260 kDa protein.

The MA1-084 immunogen is a synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.

靶标信息

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DKFZp686J1293; FLJ21220; neurofibromatosis 1; Neurofibromatosis type 1; Neurofibromatosis-related protein NF-1; Neurofibromin; Neurofibromin truncated

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基因别名: AW494271; E030030H24Rik; Nf-1; NF1; NFNS; VRNF; WSS

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UniProt ID: (Human) P21359, (Mouse) Q04690, (Rat) P97526

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Entrez Gene ID: (Human) 4763, (Mouse) 18015, (Rat) 24592

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功能

GTPase activator activity protein binding phosphatidylethanolamine binding phosphatidylcholine binding microtubule binding lipid binding syndecan binding

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