Invitrogen

NFATC4 Polyclonal Antibody

NFATC4 Antibody in Immunocytochemistry (ICC/IF)

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NFATC4 Antibody (PA1-021) in ICC/IF

Immunofluorescent analysis of NFAT3 using anti-NFAT3 polyclonal antibody (Product # PA1-021) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing NFAT3 (Product # PA1-021), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goa... View More

NFATC4 Antibody in Immunoprecipitation (IP)

NFATC4 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

NFATC4 Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-021

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

1:3000

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

3 µg

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues C R(887) D L S G F P A P P G E E P P A(902) of human NFAT3.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2267268

产品详细信息

PA1-021 detects nuclear factor of activated T-cells 3 (NFAT3) from human cells.

PA1-021 has been successfully used in Western blot immunohistochemistry, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects an ~150 kDa protein representing NFAT3 in HEK293 cells transfected with the human NFAT3 gene. Immunohistochemical staining of NFAT3 in HEK293 cells transfected with the human NFAT3 gene results in cytoplasmic staining.

The PA1-021 immunogen is a synthetic peptide corresponding to residues C R(887) D L S G F P A P P G E E P P A(902) of human NFAT3. This sequence is 94% conserved with mouse NFAT3. The PA1-021 immunizing peptide (Cat. # PEP-122) is available for use in neutralization and control experiments.

靶标信息

This protein is an adapter or scaffold protein, which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Elucidation of GPR55-Associated Signaling behind THC and LPI Reducing Effects on Ki67-Immunoreactive Nuclei in Patient-Derived Glioblastoma Cells.

Cells

Kolbe MR,Hohmann T,Hohmann U,Maronde E,Golbik R,Prell J,Illert J,Strauss C,Dehghani F

PA1-021 was used in Immunocytochemistry to investigate intracellular mechanisms leading to an altered number of Ki67+ nuclei after stimulation of GPR55 by LPI and THC.

Fri Nov 17 00:00:00 UTC 2023

Inhibition of Syk promotes chemical reprogramming of fibroblasts via metabolic rewiring and H₂ S production.

The EMBO journal

Wang W,Ren S,Lu Y,Chen X,Qu J,Ma X,Deng Q,Hu Z,Jin Y,Zhou Z,Ge W,Zhu Y,Yang N,Li Q,Pu J,Chen G,Ye C,Wang H,Zhao X,Liu Z,Zhu S

PA1-021 was used in Immunocytochemistry-immunoflourescence to show that inhibition of spleen tyrosine kinase by R406 significantly promotes mouse chemical reprogramming.

Tue Jun 01 00:00:00 UTC 2021

Transcriptional Profiling of Hypoxic Neural Stem Cells Identifies Calcineurin-NFATc4 Signaling as a Major Regulator of Neural Stem Cell Biology.

Stem cell reports

Moreno M,Fernández V,Monllau JM,Borrell V,Lerin C,de la Iglesia N

Published figure using NFATC4 polyclonal antibody (Product # PA1-021) in Immunocytochemistry

Tue Aug 11 00:00:00 UTC 2015

Transcriptional Profiling of Hypoxic Neural Stem Cells Identifies Calcineurin-NFATc4 Signaling as a Major Regulator of Neural Stem Cell Biology.

Stem cell reports

Moreno M,Fernández V,Monllau JM,Borrell V,Lerin C,de la Iglesia N

Published figure using NFATC4 polyclonal antibody (Product # PA1-021) in Immunocytochemistry

Tue Aug 11 00:00:00 UTC 2015

Nuclear factor of activated T cells (NFAT) signaling regulates PTEN expression and intestinal cell differentiation.

Molecular biology of the cell

Wang Q,Zhou Y,Jackson LN,Johnson SM,Chow CW,Evers BM

PA1-021 was used in immunohistochemistry and western blot to confirm the role of NFATc1 in PTEN expression and intestinal cell differentiation

Tue Feb 01 00:00:00 UTC 2011

Nuclear factor of activated T cells (NFAT) signaling regulates PTEN expression and intestinal cell differentiation.

Molecular biology of the cell

Wang Q,Zhou Y,Jackson LN,Johnson SM,Chow CW,Evers BM

PA1-021 was used in immunohistochemistry and western blot to confirm the role of NFATc1 in PTEN expression and intestinal cell differentiation

Tue Feb 01 00:00:00 UTC 2011

NFAT-3 is a transcriptional repressor of the growth-associated protein 43 during neuronal maturation.

The Journal of biological chemistry

Nguyen T,Lindner R,Tedeschi A,Forsberg K,Green A,Wuttke A,Gaub P,Di Giovanni S

Published figure using NFATC4 polyclonal antibody (Product # PA1-021) in Immunocytochemistry

Fri Jul 10 00:00:00 UTC 2009

NFAT-3 is a transcriptional repressor of the growth-associated protein 43 during neuronal maturation.

The Journal of biological chemistry

Nguyen T,Lindner R,Tedeschi A,Forsberg K,Green A,Wuttke A,Gaub P,Di Giovanni S

Published figure using NFATC4 polyclonal antibody (Product # PA1-021) in Immunocytochemistry

Fri Jul 10 00:00:00 UTC 2009

Phosphorylation of NFATc4 by p38 mitogen-activated protein kinases.

Molecular and cellular biology

Yang TT,Xiong Q,Enslen H,Davis RJ,Chow CW

PA1-021 was used in western blot to study the function of nuclear factor of activated T cells (NFAT) in PPARgamma gene expression and adipocyte differentiation.

Sat Jun 01 00:00:00 UTC 2002

生物信息学

蛋白别名: NF-AT3; NF-ATc4; Nuclear factor of activated T-cells, cytoplasmic 4; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4; T-cell transcription factor NFAT3

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基因别名: NF-AT3; NF-ATC4; NFAT3; NFATC4

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UniProt ID: (Human) B5B2W1

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Entrez Gene ID: (Human) 4776

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription coactivator activity protein binding transcription factor binding peroxisome proliferator activated receptor binding

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