Bethyl Laboratories

NFIC Polyclonal Antibody

NFIC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NFIC Antibody (A303-123A) in IHC (P)

Detection of mouse NFIC by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NFIC (Product # A303-123A) (Lot 1, left image) used at a dilution of 1:200 (1 µg/mL) and (Product # A303-124A) (Lot 1, right image) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

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产品信息

A303-123A

应用

建议稀释比

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免疫印迹 (WB)

1:2,000-1:10,000

免疫组化 (IHC)

1:200-1:1,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫沉淀 (IP)

2-10 µg/mg lysate

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 200 and 250 of human Nuclear Factor I/C.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.20 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS, pH 7.0 to 8.0, with 0.1% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Pig

靶标信息

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CCAAT-box-binding transcription factor; CTF; KIAA1439; NF-I/A; NF-I/B; NF-I/C; NF-I/X; NF1-A; NF1-B; NF1-C; NF1-X; NFI-A; NFI-B; NFI-C; NFI-X; NFIA; NFIB; NFIX; Nuclear factor 1 C-type; nuclear factor 1-C2; Nuclear factor 1/A; Nuclear factor 1/B; nuclear factor 1/C; Nuclear factor 1/X; Nuclear factor I/A; Nuclear factor I/B; Nuclear factor I/C; nuclear factor I/C (CCAAT-binding transcription factor); Nuclear factor I/X; TGGCA-binding protein

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基因别名: 1110019L22Rik; 1500041O16Rik; AA589446; AI746521; CTF; CTF5; NF-I; NF1-C; NFI; NFIC

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UniProt ID: (Human) P08651, (Mouse) P70255

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Entrez Gene ID: (Human) 4782, (Mouse) 18029

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region DNA binding DNA binding double-stranded DNA binding DNA-binding transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter DNA replication transcription from RNA polymerase II promoter odontogenesis of dentin-containing tooth positive regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter negative regulation of transcription, DNA-templated