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Product Details
PA5-100638
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NISCH(Accession Q9Y2I1), corresponding to amino acid residues F1189-L1239.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850144
Product Specific Information
Antibody detects endogenous levels of total NISCH.
Target Information
Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hIRAS; I-1; I-1 receptor candidate protein; I1R; I1R candidate protein; Imidazoline receptor 1; imidazoline receptor antisera selected; Imidazoline receptor antisera-selected protein; imidazoline receptor I-1; Imidazoline receptor I-1-like protein; Imidazoline-1 receptor; Imidazoline-1 receptor candidate protein; IR1; Nischarin
Gene Aliases: 1200007D05Rik; 3202002H23Rik; AW494485; hIRAS; I-1; IR1; IRAS; KIAA0975; NISCH
UniProt ID: (Human) C9J245, (Mouse) Q80TM9
Entrez Gene ID: (Human) 11188, (Rat) 306255, (Mouse) 64652
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