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NKG2AB6 Antibody (12-5897-81) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.5 µg of Mouse IgG2a k Isotype Control PE (Product # 12-4732-81) (left) or 0.5 µg of Anti-Mouse NKG2AB6 PE (right). Samples were pre-incubated with Anti-Mouse CD16/CD32 Purified (Product # 14-0161-82). Total viable cells were used for analysis.
产品信息
12-5897-81
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
16a11
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_466024
产品详细信息
Description: The 16a11 monoclonal antibody reacts with the mouse NKG2A^B6. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD95 and are responsible for recognition of non-classical MHC class I antigen Qa-1. This antibody reacts with the C57Bl/6 mouse strain, but does not react with the BALB/c or 129 mouse strains.
When co-staining with NKG2A/C/E clone 20d5, it is important to stain for NKG2A^B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently.
Applications Reported: 16a11 has been reported for use in flow cytometric analysis.
Applications Tested: The 16a11 antibody has been tested by flow cytometric analysis of C57Bl/6 (positive control) and BALB/c (negative control) mouse splenocytes and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells. The genes of NKG2 family members form a killer cell lectin-like receptor gene cluster on chromosome 12. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Cluster of Differentiation 159a; NKG2-A/NKG2-B type II integral membrane protein
基因别名: CD159a; NKG2A; NKG2B
Entrez Gene ID: (Mouse) 16641
