Invitrogen

NLGN4X (Neuroligin-4) Monoclonal Antibody (CSTEM30), Alexa Fluor™ 488, eBioscience™

NLGN4X (Neuroligin-4) Antibody in Immunocytochemistry (ICC/IF)

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NLGN4X (Neuroligin-4) Antibody (53-9158-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2a K Isotype Control (Product # 14-4724-82) (left) or 5 µg/mL Anti-Human NLGN4X (Neuroligin-4) Alexa Fluor® 488 (right). Nuclei are stained with DAPI. An iPSC colony (positive staining) and mouse embryonic fibroblasts (negative staining) are in both fields of view.

NLGN4X (Neuroligin-4) Antibody in Flow Cytometry (Flow)

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产品信息

53-9158-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

0.125 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

CSTEM30

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2716973

产品详细信息

This monoclonal antibody CSTEM30 reacts with human Neuroligin-4, X linked, also known as NLGN4X, NLGN4, Neuroligin-4, and Neuroligin 4X.

Applications Reported: This CSTEM30 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis.

Applications Tested: This CSTEM30 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human IPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.125 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

靶标信息

Neuroligin-4, X linked (also known as NLGN4X, NLGN4, Neuroligin-4, and Neuroligin 4X) is a postsynaptic cell-adhesion molecule that interacts with presynaptic cell-surface neurexins. These interactions bridge together the synaptic cleft and have an essential role in synaptic transmissions between neurons. There are 5 neuroligin genes in humans: NLGN1, NLGN2, NLGN3, NLGN4X, and NLGN4Y. Mutations in NLGN3 and NLGN4X are linked to Autism Spectrum Disorders (ASD). The expression of neuroligin-4, X-linked has been reported on human pluripotent stem cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。