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NME1 Antibody (PA5-28794) in ICC/IF
Immunofluorescence analysis of NME1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NME1 Polyclonal Antibody (Product # PA5-28794) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
产品信息
PA5-28794
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to a region within amino acids 89 and 152 of Human nm23-H1
偶联物
形式
Liquid
浓度
0.98 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2546270
产品详细信息
Recommended positive controls: 293T, Raji.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GAAD; Granzyme A activated DNase (GAAD); Granzyme A-activated DNase; GZMA activated DNase; included; Metastasis inhibition factor nm23; NDK A; NDP kinase A; NM23-H1; NM23H1B; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein
基因别名: AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NME1
UniProt ID: (Human) P15531
Entrez Gene ID: (Human) 4830
magnesium ion binding
RNA polymerase II regulatory region sequence-specific DNA binding
single-stranded DNA binding
deoxyribonuclease activity
nucleoside diphosphate kinase activity
protein binding
ATP binding
GTP binding
intermediate filament binding
protein kinase binding
identical protein binding
gamma-tubulin binding
ribosomal small subunit binding
poly(A) RNA binding
kinase
negative regulation of myeloid leukocyte differentiation
purine nucleotide metabolic process
nucleoside diphosphate phosphorylation
GTP biosynthetic process
pyrimidine nucleotide metabolic process
UTP biosynthetic process
CTP biosynthetic process
DNA metabolic process
endocytosis
lactation
negative regulation of cell proliferation
nucleoside triphosphate biosynthetic process
negative regulation of gene expression
positive regulation of neuron projection development
response to amine
nucleobase-containing small molecule interconversion
hippocampus development
response to testosterone
cellular response to drug
regulation of apoptotic process
positive regulation of DNA binding
positive regulation of epithelial cell proliferation
response to cAMP
cellular response to glucose stimulus
cellular response to fatty acid
