Invitrogen

NONO Polyclonal Antibody

NONO Antibody in Immunocytochemistry (ICC/IF)

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NONO Antibody (PA5-27408) in ICC/IF

Immunofluorescence analysis of Non-POU domain-containing octamer-binding protein was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NONO Polyclonal Antibody (Product # PA5-27408) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Ant... View More

NONO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NONO Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Non-POU domain-containing octamer-binding protein siRNA and decrease in signal intensity was observed in Western Blot application using Anti-NONO Polyclonal Antibody (Product # PA5-27408). {KD}

产品信息

PA5-27408

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 143 of Human p54/nrb

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.72 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2544884

产品详细信息

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (87%), Chicken (90%), Chimpanzee (100%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; HGNC:7871; NMT-1; NMT55; non-POU domain-containing octamer (ATGCAAAT) binding protein; Non-POU domain-containing octamer-binding protein; non-POU-domain-containing; NonO protein; p54(nrb); protein phosphatase 1, regulatory subunit 114

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基因别名: AA407051; AV149256; MRXS34; NMT55; nonA; NONO; NRB54; P54; P54NRB; PPP1R114

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UniProt ID: (Human) Q15233, (Mouse) Q99K48

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Entrez Gene ID: (Human) 4841, (Mouse) 53610

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