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NOTUM Antibody (PA5-95811) in WB
Western blot analysis of NOTUM in extracts of various cell lines. Samples were incubated with NOTUM Polyclonal antibody (Product # PA5-95811) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-95811
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human NOTUM (NP_8485883)
Conjugate
Form
Liquid
Concentration
1.23 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807613
Product Specific Information
Immunogen sequence: SEGRKTWRRR GQQPPPPPRT EAAPAAGQPV ESFPLDFTAV EGNMDSFMAQ VKSLAQSLYP CSAQQLNEDL RLHLLLNTSV TCNDGSPAGY YLKESRGSRR WLLFLEGGWY CFNRENCDSR YDTMRRLMSS RDWPRTRTGT GILSSQPEEN PYWWNANMVF IPYCSSDVWS GASSKSEKNE YAFMGALIIQ EVVRELLGRG LSGAKVLLLA GSSAGGTGVL LNVDRVAEQL EKLGYPAIQV RGLADSGWFL DNKQYRHTDC VDTITCAPTE AIRRGIRYWN GVVPERCRRQ FQEGEEWNCF F; Positive Samples: BxPC-3, HepG2
Target Information
Genetic studies in Drosophila have revealed a novel secreted antagonist for Wingless (Wg) signaling, referred to as Notum/Wingful (Wf). Wf/Notum encodes a protein of 430 amino acid residues, which is related to plant pectin acetylesterases of the a/beta-hydrolase superfamily. The main target of Notum action appears to be cell surface proteoglycans Dally and/or Dally-like (Dlp). Notum governs the extracellular Wg morphogen distribution via Dally and Dlp. Also, like some vertebrate Wnt antagonists, notum expression is activated by Wg signaling, revealing the existence of a feedback control mechanism in which Wg modifies its own gradient distribution. In vertebrate, proteoglycans related to Dally and Dlp likely play important roles in Wnt signaling which encodes a glypican that is required for Wnt-11 signaling. A single ortholog of Notum exists in the mouse and the human genome, implying that a similar regulatory mechanism may exist in vertebrates.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [Wnt protein] O-palmitoleoyl-L-serine hydrolase; hNOTUM; notum pectinacetylesterase homolog; Palmitoleoyl-protein carboxylesterase NOTUM; protein notum homolog
Gene Aliases: hNOTUM; NOTUM; OK/SW-CL.30
UniProt ID: (Human) Q6P988
Entrez Gene ID: (Human) 147111
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