Invitrogen

NOV Polyclonal Antibody, Biotin, PeproTech®

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NOV Antibody (500-P257BT-25UG) in WB

Western Blot: To detect hNOV by Western Blot analysis NOV Polyclonal Antibody, Biotin (Product # 500-P257BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNOV is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P257BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived, 36.2 kDa Recombinant Human NOV

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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930108

产品详细信息

AA Sequence of recombinant protein: MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NOV. Anti-Human NOV-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hNOV by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NOV (500-P257) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hNOV.

Western Blot: To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNOV is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions

500-P257BT-1MG will be provided as 2 x 500 µg

靶标信息

NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm's tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and cell migration by signaling through specific cell-surface integrins, and by binding to heparin sulfate proteoglycans and to fibulin 1C. NOV has also been reported to exert proangiogenic activities.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CCN family member 3; Cellular communication network factor 3; IBP-9; IGF-binding protein 9; Insulin-like growth factor-binding protein 9; Nephro blastoma-overexpressed gene protein homolog; nephroblastoma-overexpressed gene protein homolog; NovH; Protein NOV homolog

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基因别名: CCN3; IBP-9; IGFBP-9; IGFBP9; NOV; NOVH

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UniProt ID: (Human) P48745

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Entrez Gene ID: (Human) 4856

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功能

Notch binding integrin binding protein binding insulin-like growth factor binding growth factor activity heparin binding growth factor

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