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NOX1 Antibody (PA5-79752) in IHC (P)
Immunohistochemistry (Paraffin) analysis of NOX1 in paraffin-embedded section of rat colon tissue using NOX1 Polyclonal Antibody (Product # PA5-79752). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at 4°C. Per... View More
Product Details
PA5-79752
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of rat NOX1 (417-431aa WYKFQRAHNKLKTQK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746867
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue. IHC: rat colon tissue.
Target Information
Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv. NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H (+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. NOX1 have the potential to be effective treatments for a range of ischemic diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GP91 phox homolog; Mitogenic oxidase 1; MOX-1; MOX1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase 1; NADPH oxidase 1 alpha; NOH-1; NOX-1
Gene Aliases: GP91-2; MOX1; NOH-1; NOH1; Nox-1; Nox1; NOX1a; NOX1alpha
UniProt ID: (Rat) Q9WV87, (Mouse) Q8CIZ9
Entrez Gene ID: (Rat) 114243, (Mouse) 237038
protein binding
superoxide-generating NADPH oxidase activity
voltage-gated proton channel activity
metal ion binding
Rac GTPase binding
N-ethylmaleimide reductase activity
oxidoreductase activity
reduced coenzyme F420 dehydrogenase activity
sulfur oxygenase reductase activity
malolactic enzyme activity
NADPH:sulfur oxidoreductase activity
epoxyqueuosine reductase activity
response to reactive oxygen species
angiogenesis
regulation of systemic arterial blood pressure by renin-angiotensin
superoxide metabolic process
signal transduction
positive regulation of cell proliferation
positive regulation of vascular endothelial growth factor production
proton transport
cell migration
extracellular matrix organization
superoxide anion generation
hydrogen peroxide metabolic process
positive regulation of MAPK cascade
positive regulation of integrin biosynthetic process
positive regulation of JNK cascade
positive regulation of smooth muscle cell proliferation
intracellular pH elevation
oxidation-reduction process
cellular response to hyperoxia
oxygen metabolic process
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
cellular stress response to acidic pH
positive regulation of reactive oxygen species metabolic process
transport
regulation of ion transmembrane transport
cation transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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