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NOX3 Antibody (PA5-38036) in ICC/IF
Immunofluorescent analysis of Nox3 (Violet) in mouse bone marrow cells (Up). The cells were fixed with 2% paraformaldehyde in PBS for 30 minutes at RT, permeabilized with 0.5% Triton X-100 in PBS for 30 minutes at RT, and blocked with blocking buffer for 4 hours in cold room. Cells were stained with rabbit polyclonal anti-Nox3 antibody (Product # PA5-38036) at a dilution of 1:200 in blocking buffer overnight in cold room, wash 5 times with 1 xPBS, incubated with goat anti-rabbit IgG antibody conjugated with Cy5 (Product # A10523) at a dilu... View More
产品信息
PA5-38036
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
15 amino acid peptide near the amino terminus of human NOX3
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2554640
产品详细信息
PA5-38036 detects Nox3 in mouse bone marrow and humanG-CSF mobilized CD34+ cells in immunofluorescence analysis.
A recommended positive control is 293 cell lysate.
NOX3 is not expected to cross-react with other NOX proteins.
靶标信息
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide. NOX3 is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith. It has been suggested that NOX3 is activated by the Transient Receptor Potential Vanilloid 1 (TRPV1), and this activity causes increased levels of reactive oxygen species in the inner ear, which in turn leads to STAT1-mediated inflammation and hearing loss.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GP91-3; gp91phox homolog 3; head-tilt; Mitogenic oxidase 2; MOX-2; NADPH oxidase 3; NADPH oxidase catalytic subunit-like 3; NAPDH oxidase 3; NOX-3
基因别名: GP91-3; het; MOX-2; MOX2; nmf250; NOX3
UniProt ID: (Human) Q9HBY0, (Mouse) Q672J9
Entrez Gene ID: (Human) 50508, (Mouse) 224480
