Invitrogen

NOX4 Recombinant Rabbit Monoclonal Antibody (SY0214)

NOX4 Antibody in Immunocytochemistry (ICC/IF)

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NOX4 Antibody (MA5-32090) in ICC/IF

Immunocytochemical analysis of NOX4 in A549 cells using a NOX4 Monoclonal antibody (Product # MA5-32090) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

NOX4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NOX4 Antibody in Immunohistochemistry (IHC)

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产品信息

MA5-32090

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100

功能检测 (Functional)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SY0214

抗原

Recombinant protein within Human NOX4 aa 460-578

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809383

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Oxygen sensing is essential for homeostasis in all aerobic organisms. A phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes, with resultant antimicrobial activity, has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. NOX4 has a role as a redox messenger in the activation of intracellular signaling pathways leading (or contributing) to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. Data suggests that NOX4 provides a novel link between the insulin receptor and the generation of cellular reactive oxygen species that enhances insulin signal transduction.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

MA5-32090 was used in Western Blotting to demonstrate TCam-2 spheroid sensitivity to acute s-microgravity exposure and indicate the capability of these cells to trigger compensatory mechanisms that allow them to overcome the exposure to altered gravitational force.

Sat Aug 19 00:00:00 UTC 2023

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

Sat Aug 19 00:00:00 UTC 2023

Repetitive Transcranial Magnetic Stimulation Reduces Neuronal Loss and Neuroinflammation in Parkinson?s Disease Through the miR-195a-5p/CREB Axis.

Turkish neurosurgery

Sun L,Wang F,Han J,Bai L,DU J

MA5-32090 was used in Western Blotting to find that miR-195a-5p suppression enhanced cognitive and motor functions in PD mice.

Tue Apr 04 00:00:00 UTC 2023

Role of the OTUB1/IRF7/NOX4 axis in oxidative stress injury and inflammatory responses in mice with Parkinson's disease.

Psychogeriatrics : the official journal of the Japanese Psychogeriatric Society

Xing X,Xu F,Wang Y,Liu H

MA5-32090 was used in Western Blotting to conclude that OTUB1-mediated deubiquitination stabilized IRF7 and upregulated NOX4 expression, thereby promoting oxidative stress injury (OSI) and inflammatory responses in Parkinson's disease (PD) mice.

Sun Jan 01 00:00:00 UTC 2023

Oxidative stress and inflammation distinctly drive molecular mechanisms of diastolic dysfunction and remodeling in female and male heart failure with preserved ejection fraction rats.

Frontiers in cardiovascular medicine

Zhazykbayeva S,Hassoun R,Herwig M,Budde H,Kovács Á,Mannherz HG,El-Battrawy I,Tóth A,Schmidt WE,Mügge A,Hamdani N

MA5-32090 was used in Western Blotting to suggest that oxidative stress and inflammation distinctly drive diastolic dysfunction and remodeling in female and male rats with HFpEF and that the sex-dependent mechanisms contribute to HF pathology.

Sat Jul 01 00:00:00 UTC 2023

IL11 stimulates ERK/P90RSK to inhibit LKB1/AMPK and activate mTOR initiating a mesenchymal program in stromal, epithelial, and cancer cells.

iScience

Widjaja AA,Viswanathan S,Wei Ting JG,Tan J,Shekeran SG,Carling D,Lim WW,Cook SA

MA5-32090 was used in Functional Assay to identify the IL11/mTOR axis as a signaling commonality in stromal, epithelial, and cancer cells and reveal a shared IL11-driven mesenchymal program across cell types.

Fri Aug 19 00:00:00 UTC 2022

Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH.

Nature communications

Dong J,Viswanathan S,Adami E,Singh BK,Chothani SP,Ng B,Lim WW,Zhou J,Tripathi M,Ko NSJ,Shekeran SG,Tan J,Lim SY,Wang M,Lio PM,Yen PM,Schafer S,Cook SA,Widjaja AA

MA5-32090 was used in Western Blot to show that IL11 modulates hepatocyte metabolism and suggests a mechanism for NAFLD to NASH transition.

Mon Jan 04 00:00:00 UTC 2021

Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH.

Nature communications

Dong J,Viswanathan S,Adami E,Singh BK,Chothani SP,Ng B,Lim WW,Zhou J,Tripathi M,Ko NSJ,Shekeran SG,Tan J,Lim SY,Wang M,Lio PM,Yen PM,Schafer S,Cook SA,Widjaja AA

MA5-32090 was used in Western Blot to show that IL11 modulates hepatocyte metabolism and suggests a mechanism for NAFLD to NASH transition.

Mon Jan 04 00:00:00 UTC 2021

NOX1 Inhibition Attenuates Kidney Ischemia-Reperfusion Injury via Inhibition of ROS-Mediated ERK Signaling.

International journal of molecular sciences

Jung HY,Oh SH,Ahn JS,Oh EJ,Kim YJ,Kim CD,Park SH,Kim YL,Cho JH

MA5-32090 was used in Immunohistochemistry to identify the effect of NOX1 inhibition on the recovery from IRI through ROS suppression and related mechanisms.

Mon Sep 21 00:00:00 UTC 2020

NOX1 Inhibition Attenuates Kidney Ischemia-Reperfusion Injury via Inhibition of ROS-Mediated ERK Signaling.

International journal of molecular sciences

Jung HY,Oh SH,Ahn JS,Oh EJ,Kim YJ,Kim CD,Park SH,Kim YL,Cho JH

MA5-32090 was used in Immunohistochemistry to identify the effect of NOX1 inhibition on the recovery from IRI through ROS suppression and related mechanisms.

Mon Sep 21 00:00:00 UTC 2020

生物信息学

蛋白别名: Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX-1; KOX1; NADPH oxidase 4; NOX 4; NOX-4; Renal NAD(P)H-oxidase; Superoxide-generating NADPH oxidase 4

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基因别名: AI648021; KOX; KOX-1; NOX4; RENOX

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UniProt ID: (Human) Q9NPH5, (Rat) Q924V1, (Mouse) Q9JHI8

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Entrez Gene ID: (Human) 50507, (Rat) 85431, (Mouse) 50490

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功能