Invitrogen

NPR1 Polyclonal Antibody

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NPR1 Antibody (PA5-29049) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) and tissue extracts of Rat Adipose (Lane 2). The blot was probed with Anti-NPR1 Polyclonal Antibody (Product # PA5-29049, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 118 kDa band corresponding to NPR1 was detected in the cell line and tissue tested. An uncharacterized band was also observed at ~20kDa in th... View More

NPR1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

NPR1 Antibody in Immunohistochemistry (IHC)

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NPR1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with NPR1 siRNA and reduction of signal was observed in Western Blot using NPR1 Polyclonal Antibody (Product # PA5-29049). {KD}

产品信息

PA5-29049

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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产品规格

种属反应

Human, Rabbit, Rat

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 640 and 916 of Human NPR-A

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2546525

产品详细信息

Recommended positive controls: HL-60 membrane extracts.

Predicted reactivity: Mouse (95%), Rat (95%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms. The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A and type B. Also see NPR3, which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides and BNP, respectively).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Combination of an Angiotensin II Receptor and a Neprilysin Inhibitor Attenuates Liver Fibrosis by Preventing Hepatic Stellate Cell Activation.

Biomedicines

Suzuki J,Kaji K,Nishimura N,Kubo T,Tomooka F,Shibamoto A,Iwai S,Tsuji Y,Fujinaga Y,Kitagawa K,Namisaki T,Akahane T,Yoshiji H

PA5-29049 was used in Western Blotting to assess the effects of SAC/VAL in carbon tetrachloride (CCl4)-induced murine liver fibrosis as well as the in vitro phenotypes of hepatic stellate cell (HSC).

Thu Apr 27 00:00:00 UTC 2023

VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.

Cells

Zhang X,Zhou K,Tian K,Zhu Q,Liu W,Liu Z,An X,Tian C,Li Y,Lu F,Sun F,Zha D

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Immunohistochemistry

Wed Nov 23 00:00:00 UTC 2022

VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.

Cells

Zhang X,Zhou K,Tian K,Zhu Q,Liu W,Liu Z,An X,Tian C,Li Y,Lu F,Sun F,Zha D

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Immunohistochemistry

Wed Nov 23 00:00:00 UTC 2022

Atrial Natriuretic Peptide Promotes Neurite Outgrowth and Survival of Cochlear Spiral Ganglion Neurons in vitro Through NPR-A/cGMP/PKG Signaling.

Frontiers in cell and developmental biology

Sun F,Zhou K,Tian KY,Zhang XY,Liu W,Wang J,Zhong CP,Qiu JH,Zha DJ

PA5-29049 was used in Immunohistochemistry to study how atrial natriuretic peptide (ANP) could support and attract neurite outgrowth of spiral ganglion neurons (SGNs) and possess a high capacity to improve neuronal survival of SGNs against glutamate-induced excitotoxicity by triggering the natriuretic peptide receptor (NPR-A)/cyclic guanosine-3',5'-monophosphate (cGMP)/PKG pathway.

Sat Jul 17 00:00:00 UTC 2021

Natriuretic Peptides Regulate Prostate Cells Inflammatory Behavior: Potential Novel Anticancer Agents for Prostate Cancer.

Biomolecules

Mezzasoma L,Talesa VN,Costanzi E,Bellezza I

PA5-29049 was used in Western Blot to analyze the contribution of both Atrial Natriuretic Peptides (ANP) and B-Type Natriuretic Peptide (BNP) in the regulation of the inflammatory behavior in prostate cancerous and non-cancerous cell lines.

Tue May 25 00:00:00 UTC 2021

Natriuretic Peptides Regulate Prostate Cells Inflammatory Behavior: Potential Novel Anticancer Agents for Prostate Cancer.

Biomolecules

Mezzasoma L,Talesa VN,Costanzi E,Bellezza I

Tue May 25 00:00:00 UTC 2021

An approach using Caenorhabditis elegans screening novel targets to suppress tumour cell proliferation.

Cell proliferation

Mao YQ,Han SF,Zhang SL,Zhang ZY,Kong CY,Chen HL,Li ZM,Cai PR,Han B,Wang LS

PA5-29049 was used in Immunohistochemistry-immunofluorescence to perform dauer gene screening in combination with tumour-suppressing test in glp-1(-) mutants provided a useful approach to find potential targets for tumour therapy via suppressing tumour cell proliferation and rewiring tumour cell metabolism.

Mon Jun 01 00:00:00 UTC 2020

An approach using Caenorhabditis elegans screening novel targets to suppress tumour cell proliferation.

Cell proliferation

Mao YQ,Han SF,Zhang SL,Zhang ZY,Kong CY,Chen HL,Li ZM,Cai PR,Han B,Wang LS

Mon Jun 01 00:00:00 UTC 2020

Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner.

Neural plasticity

Sun F,Zhou K,Tian KY,Wang J,Qiu JH,Zha DJ

PA5-29049 was used in Immunohistochemistry to investigate the expression patterns of ANP and its receptors in the cochlear SG and dissociated SGNs and determined the influence of ANP on neurite outgrowth in vitro by using organotypic SG explants and dissociated SGN cultures from postnatal rats.

Wed Sep 29 00:00:00 UTC 2021

Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner.

Neural plasticity

Sun F,Zhou K,Tian KY,Wang J,Qiu JH,Zha DJ

Wed Sep 29 00:00:00 UTC 2021

ANP and BNP Exert Anti-Inflammatory Action via NPR-1/cGMP Axis by Interfering with Canonical, Non-Canonical, and Alternative Routes of Inflammasome Activation in Human THP1 Cells.

International journal of molecular sciences

Mezzasoma L,Talesa VN,Romani R,Bellezza I

PA5-29049 was used in Western Blotting to aim to decipher the molecular mechanism underlying NPs effects on THP-1 cells stimulated with lipopolysaccharide (LPS) + ATP. Involvement of cGMP and PKG-I were assessed pre-treating THP-1 cells with the membrane-permeable analogue, 8-Br-cGMP, and the specific inhibitor KT-5823, respectively.

Tue Dec 22 00:00:00 UTC 2020

ANP and BNP Exert Anti-Inflammatory Action via NPR-1/cGMP Axis by Interfering with Canonical, Non-Canonical, and Alternative Routes of Inflammasome Activation in Human THP1 Cells.

International journal of molecular sciences

Mezzasoma L,Talesa VN,Romani R,Bellezza I

Tue Dec 22 00:00:00 UTC 2020

生物信息学

蛋白别名: ANP-A; ANPR-A; atrial natriuretic peptide A-type receptor; Atrial natriuretic peptide receptor 1; atrial natriuretic peptide receptor A; Atrial natriuretic peptide receptor type A; atrionatriuretic peptide receptor A; GC-A; Guanylate cyclase A; natriuretic peptide A type receptor; natriuretic peptide receptor A; natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A); NPR-A; testicular tissue protein Li 20

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基因别名: ANPa; ANPRA; Gca; GUC2A; GUCY2A; NPR-A; NPR1; NPRA

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UniProt ID: (Human) P16066, (Rat) P18910

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Entrez Gene ID: (Rabbit) 100343663, (Human) 4881, (Rat) 24603

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功能