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NPR1 Antibody (PA5-99835) in ICC/IF
Immunocytochemistry analysis of NPR1 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NPR1 polyclonal antibody (Product # PA5-99835) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-99835
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NPR1(Accession P16066), corresponding to amino acid residues R439-V489.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2818768
产品详细信息
Antibody detects endogenous levels of total NPR1.
靶标信息
Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms. The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A and type B. Also see NPR3, which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides and BNP, respectively).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ANP-A; ANPR-A; atrial natriuretic peptide A-type receptor; Atrial natriuretic peptide receptor 1; atrial natriuretic peptide receptor A; Atrial natriuretic peptide receptor type A; atrionatriuretic peptide receptor A; GC-A; Guanylate cyclase A; guanylyl cyclase-A; natriuretic peptide A type receptor; natriuretic peptide receptor A; natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A); Nppa receptor; NPR-A; testicular tissue protein Li 20
基因别名: AI893888; ANPa; ANPRA; GC-A; Gca; GUC2A; GUCY2A; NPR-A; NPR1; NPRA; Pndr
UniProt ID: (Human) P16066, (Mouse) P18293, (Rat) P18910
Entrez Gene ID: (Human) 4881, (Mouse) 18160, (Rat) 24603
adenylate cyclase activity
guanylate cyclase activity
protein kinase activity
ATP binding
GTP binding
G-protein coupled peptide receptor activity
natriuretic peptide receptor activity
peptide hormone binding
protein kinase binding
hormone binding
nucleotide binding
transferase activity, transferring phosphorus-containing groups
lyase activity
phosphorus-oxygen lyase activity
protein binding
guanylate cyclase
cGMP biosynthetic process
protein phosphorylation
cell surface receptor signaling pathway
receptor guanylyl cyclase signaling pathway
G-protein coupled receptor signaling pathway
body fluid secretion
regulation of blood pressure
negative regulation of angiogenesis
negative regulation of cell growth
positive regulation of cGMP biosynthetic process
intracellular signal transduction
positive regulation of urine volume
positive regulation of renal sodium excretion
regulation of vasodilation
dopamine metabolic process
regulation of vascular permeability
negative regulation of smooth muscle cell proliferation
regulation of cardiac conduction
cyclic nucleotide biosynthetic process
regulation of blood vessel size
